Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Strupp, M; Quasthoff, S; Mitrovic, N; Grafe, P.
Glutathione accelerates sodium channel inactivation in excised rat axonal membrane patches.
Pflugers Arch. 1992; 421(2-3):283-285
Doi: 10.1007/BF00374840
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- Co-Autor*innen der Med Uni Graz
- Quasthoff Stefan
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- Abstract:
- The effects of glutathione were studied on the gating behaviour of sodium channels in membrane patches of rat axons. Depolarizing pulses from -120 to -40 mV elicited sodium currents of up to 500 pA, indicating the simultaneous activation of up to 250 sodium channels. Inactivation of these channels in the excised, inside-out configuration was fitted by two time constants (tau h1 = 0.81 ms; tau h2 = 5.03 ms) and open time histograms at 0 mV revealed a biexponential distribution of channel openings (tau short = 0.28 ms; tau long = 3.68 ms). Both, the slow time constant of inactivation and the long lasting single channel openings disappeared after addition of the reducing agent glutathione (2-5 mM) to the bathing solution. Sodium channels of excised patches with glutathione present on the cytoplasmatic face of the membrane had inactivation kinetics similar to channels recorded in the cell-attached configuration. These observations indicate that redox processes may contribute to the gating of axonal sodium channels.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Axons - drug effects
- Glutathione - pharmacology
- Kinetics - pharmacology
- Male - pharmacology
- Membranes - metabolism
- Oxidation-Reduction - metabolism
- Rats - metabolism
- Rats, Inbred Strains - metabolism
- Sodium Channels - drug effects
- Find related publications in this database (Keywords)
- SODIUM CHANNEL
- AXON
- GLUTATHIONE
- METABOLISM
- REDUCING AGENT
- CYSTEINE
- DISULFIDE BRIDGES