Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Maly, K; Strese, K; Kampfer, S; Ueberall, F; Baier, G; Ghaffari-Tabrizi, N; Grunicke, HH; Leitges, M.
Critical role of protein kinase C alpha and calcium in growth factor induced activation of the Na(+)/H(+) exchanger NHE1.
FEBS Lett. 2002; 521(1-3):205-210
Doi: 10.1016/S0014-5793(02)02867-3
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- Co-Autor*innen der Med Uni Graz
- Ghaffari Tabrizi-Wizsy Nassim
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- Abstract:
- The ubiquitously expressed Na(+)/H(+) exchanger (NHE1) plays an important role in the regulation of the intracellular pH. Induction of NHE activity by phorbol esters and inhibition of growth factor-mediated stimulation of the NHE by protein kinase C (PKC) inhibitors suggest an implication of PKCs in the regulation of the NHE. Expression of PKC isotype-specific dominant negative and constitutively active mutants or downregulation of PKC by isotype-specific antisense oligonucleotides revealed that stimulation by epidermal growth factor (EGF) or phorbol ester of the NHE in NIH3T3 cells is a PKC(alpha)-specific effect. Elevation of cytoplasmic calcium by a Ca(2+) ionophore or thapsigargin causes a growth factor-independent stimulation of the NHE predominantly mediated by calcium/calmodulin kinase II. It is concluded that in NIH3T3 cells overexpressing the EGF receptor (EGFR6 cells), EGF requires cPKC(alpha) for the activation of the NHE, while calcium/calmodulin-dependent kinases are essential in thapsigargin induced stimulation of the NHE.
- Find related publications in this database (using NLM MeSH Indexing)
- 3T3 Cells -
- Animals -
- Calcium - metabolism
- Enzyme Inhibitors - pharmacology
- Epidermal Growth Factor - metabolism
- Isoenzymes - biosynthesis
- Mice - biosynthesis
- Mitogen-Activated Protein Kinases - metabolism
- Protein Kinase C - biosynthesis
- Protein Kinase C-alpha - biosynthesis
- Protein Kinase C-delta - biosynthesis
- Protein Kinase C-epsilon - biosynthesis
- Sodium-Hydrogen Antiporter - biosynthesis
- Thapsigargin - pharmacology
- Find related publications in this database (Keywords)
- Na+/H+ exchanger
- NHE1
- protein kinase C isotype
- calcium
- epidermal growth factor
- NIH3T3