Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wiltschke, C; Krainer, M; Nanut, M; Wagner, A; Linkesch, W; Zielinski, CC.
In vivo administration of granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor increases neutrophil oxidative burst activity.
J INTERFERON CYTOKINE RES. 1995; 15(3): 249-253.
Doi: 10.1089/jir.1995.15.249
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- Co-Autor*innen der Med Uni Graz
- Linkesch Werner
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- Abstract:
- The influence of CSF therapy on the superoxide (O2-) releasing capacity in response to N-formyl-methionyl-leucyl-phenylalanine (FMLP) of neutrophils from 32 patients with testicular cancer receiving high-dose chemotherapy followed by autologous bone marrow transplantation (ABMT) was assessed: 8 patients were treated as control group without CSF therapy, 12 patients received GM-CSF, and 12 patients received G-CSF. To monitor the kinetics of the respiratory burst, leukocytes were collected before initiation of chemotherapy and ABMT, during CSF administration on days 1 and 3 after leukocyte recovery, and 7 days after leukocyte recovery (controls) or 3 days after the end of CSF therapy. Neutrophils from patients who received GM-CSF showed a significantly higher superoxide anion release compared with control patients (p < 0.001). O2- production in these patients was higher than that achieved by in vitro preincubation of neutrophils from control patients. Increased burst activity was seen only during infusion of GM-CSF and returned to pretherapeutic values after the end of GM-CSF administration. A similar but less pronounced increase was seen in patients who received G-CSF. In vitro preincubation of neutrophils from the same patients with GM-CSF, G-CSF, or TNF showed that O2- production by neutrophils from patients receiving GM-CSF could not be further enhanced, whereas O2- production by neutrophils derived from patients receiving G-CSF could be further augmented by TNF but not by GM-CSF. Interestingly, neutrophils from patients treated with GM-CSF but not those with G-CSF therapy retained a higher response to in vitro stimulation with GM-CSF or TNF after the end of CSF administration.(ABSTRACT TRUNCATED AT 250 WORDS)
- Find related publications in this database (using NLM MeSH Indexing)
- Bone Marrow Transplantation -
- Case-Control Studies -
- Combined Modality Therapy -
- Granulocyte Colony-Stimulating Factor - pharmacology
- Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
- Humans - pharmacology
- Male - pharmacology
- N-Formylmethionine Leucyl-Phenylalanine - pharmacology
- Neutrophil Activation - pharmacology
- Neutrophils - metabolism
- Respiratory Burst - metabolism
- Superoxides - metabolism
- Testicular Neoplasms - metabolism
- Tumor Necrosis Factor-alpha - pharmacology