Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wiltschke, C; Krainer, M; Wagner, A; Linkesch, W; Zielinski, CC.
Influence of in vivo administration of GM-CSF and G-CSF on monocyte cytotoxicity.
EXP HEMATOL. 1995; 23(5): 402-406.
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Linkesch Werner
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- Abstract:
- The influence of colony-stimulating factors (CSFs) on the monocyte functions of 32 patients with refractory testicular cancer receiving high-dose chemotherapy followed by autologous bone marrow transplantation (ABMT) was tested. Eight patients were treated as a control group without CSF therapy, 12 patients received recombinant human granulocyte-macrophage-CSF (rhGM-CSF), and 12 patients received recombinant human granulocyte-CSF (rhG-CSF). For the assessment of monocyte activation induced by CSF expression of major histocompatibility complex (MHC) class I and II antigens, production of tumor necrosis factor (TNF) and monocyte-mediated cytotoxicity against tumor cell targets were chosen. Monocytes from patients with GM-CSF therapy showed a significant increase in MHC class I and II antigen expression as compared to patients without CSF treatment (p < 0.001). A significant increase in the expression of MHC class I was seen in monocytes from patients under G-CSF treatment, whereas no change of class II antigens was noticed. Production of TNF and monocyte-mediated cytotoxicity against U937 tumor cells was significantly increased in monocytes derived from patients receiving GM-CSF, as compared to those from the control group, while no effect was detectable in monocytes from patients with G-CSF therapy. However, after in vitro stimulation with interferon-gamma (IFN-gamma), monocytes derived from GM-CSF as well as from G-CSF treated patients responded with a significantly higher TNF-production and cytotoxicity than monocytes from control patients.
- Find related publications in this database (using NLM MeSH Indexing)
- Antineoplastic Combined Chemotherapy Protocols - therapeutic use
- Bone Marrow Transplantation - therapeutic use
- Cells, Cultured - therapeutic use
- Combined Modality Therapy - therapeutic use
- Comparative Study - therapeutic use
- Cytotoxicity, Immunologic - drug effects
- Granulocyte Colony-Stimulating Factor - pharmacology
- Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
- HLA Antigens - analysis
- Humans - analysis
- Interferon Type II - pharmacology
- Male - pharmacology
- Monocytes - drug effects
- Testicular Neoplasms - blood
- Find related publications in this database (Keywords)
- GM-CSF
- G-CSF
- MONOCYTE
- CYTOTOXICITY
- MHC ANTIGENS