Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Baciulis, V; Luthy, C; Hofer, G; Toplak, H; Wiesmann, UN; Oetliker, OH.
Specific and non specific stimulation of prostaglandin release by human skin fibroblasts in culture.--Are changes of membrane fluidity involved?
Prostaglandins. 1992; 43(3):293-304
Doi: 10.1016/0090-6980(92)90097-D
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- Co-Autor*innen der Med Uni Graz
- Toplak Hermann
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- Abstract:
- In order to study a bidirectional relationship between changes of membrane fluidity and prostaglandin synthesis, the arachidonic acid cascade was stimulated in cultured human skin fibroblasts by unspecific stimuli (hypotonicity, low calcium concentrations) and by the specific stimulus, bradykinin. Fluorescence anisotropy of trimethylammoniumdiphenylhexatriene was used to measure membrane fluidity in cell monolayers. Hypotonicity or low calcium concentrations induce membrane fluidisation and prostaglandin synthesis. However, after specific stimulation of prostaglandins with bradykinin (at normocalcic and isotonic conditions) a rigidification of plasma membranes was observed in living cells. Fluidisation of membranes and bradykinin activate phospholipase A2 and induce prostaglandin synthesis. Although in cell membrane preparations increased phospholipase A2 activity leads to fluidisation, in our model a membrane fluidisation was not observed after stimulation of phospholipase with bradykinin. This suggests that in living cells a fluidizing effect of lysolecithin resulting from phospholipase A2 activation may be rapidly counteracted by its removal. A decrease of phosphatidylcholin content and consequently a rigidification of the membrane may ensue. Thus, the cell culture model using two different ways of stimulating phospholipase activity, helps to define the directional relationship between changes of membrane fluidity and activation of phospholipase and the arachidonic acid cascade in living human cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Bradykinin - pharmacology
- Calcium - administration and dosage
- Cells, Cultured - administration and dosage
- Diphenylhexatriene - analogs and derivatives
- Fibroblasts - drug effects
- Fluorescence Polarization - drug effects
- Fluorescent Dyes - drug effects
- Humans - drug effects
- Membrane Fluidity - physiology
- Osmolar Concentration - physiology
- Prostaglandins - biosynthesis