Medizinische Universität Graz - Research portal
Selected Publication:
Weitgasser, U; Haller, EM; El-Shabrawi, Y.
Evaluation of polymerase chain reaction for the detection of adenoviruses in conjunctival swab specimens using degenerate primers in comparison with direct immunofluorescence.
OPHTHALMOLOGICA 2002 216: 329-332.
Doi: 10.1159/000066175
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- El-Shabrawi Yosuf
- Haller-Schober Eva-Maria
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- Abstract:
- PURPOSE: To investigate a rapid and sensitive polymerase chain reaction (PCR)-based assay for the detection of adenoviral infections in conjunctival swabs. METHODS: Degenerate primers derived from the adenoviral hexon gene were designed for amplification of a 302-bp product by PCR. In 15 ocular swab specimens, using a simple sample preparation method, PCR was compared with a commercially available direct immunofluorescence test. RESULTS: Fourteen samples of patients with clinically diagnosed adenoviral keratoconjunctivitis, when screened by direct immunofluorescence, were positive for adenoviral protein. In all samples positive by immunofluorescence, the 302-bp product was amplifiable. The sample negative by direct immunofluorescence was positive using PCR. CONCLUSION: PCR using degenerate primers proved to have several advantages over current diagnostic techniques. It offers considerable improvement in sensitivity over immunoassays and speed over tissue culture isolation and is a highly potential tool for the diagnosis of adenoviral ocular infections.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenoviridae Infections - diagnosis
- Adenoviruses, Human - genetics
- Comparative Study - genetics
- Conjunctiva - virology
- Conjunctivitis, Viral - diagnosis
- DNA Primers - diagnostic use
- DNA, Viral - analysis
- Fluorescent Antibody Technique, Direct - analysis
- Humans - analysis
- Keratitis - virology
- Polymerase Chain Reaction - standards
- Find related publications in this database (Keywords)
- adenovirus
- conjunctivitis
- conjunctival swabs
- polymerase chain reaction
- direct immunofluorescence test