Medizinische Universität Graz - Research portal
Selected Publication:
Pfeifhofer, C; Kofler, K; Gruber, T; Tabrizi, NG; Lutz, C; Maly, K; Leitges, M; Baier, G.
Protein kinase C theta affects Ca2+ mobilization and NFAT cell activation in primary mouse T cells.
J Exp Med. 2003; 197(11):1525-1535
Doi: 10.1084/jem.20020234
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- Co-authors Med Uni Graz
- Ghaffari Tabrizi-Wizsy Nassim
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- Abstract:
- Protein kinase C (PKC)theta is an established component of the immunological synapse and has been implicated in the control of AP-1 and NF-kappaB. To study the physiological function of PKCtheta, we used gene targeting to generate a PKCtheta null allele in mice. Consistently, interleukin 2 production and T cell proliferative responses were strongly reduced in PKCtheta-deficient T cells. Surprisingly, however, we demonstrate that after CD3/CD28 engagement, deficiency of PKCtheta primarily abrogates NFAT transactivation. In contrast, NF-kappaB activation was only partially reduced. This NFAT transactivation defect appears to be secondary to reduced inositol 1,4,5-trisphosphate generation and intracellular Ca2+ mobilization. Our finding suggests that PKCtheta plays a critical and nonredundant role in T cell receptor-induced NFAT activation.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Base Sequence -
- Calcium Signaling -
- Cells, Cultured -
- DNA - genetics
- DNA-Binding Proteins - genetics
- Isoenzymes - deficiency
- Lymphocyte Activation - deficiency
- Mice - deficiency
- Mice, Knockout - deficiency
- NF-kappa B - metabolism
- NFATC Transcription Factors - metabolism
- Nuclear Proteins - metabolism
- Protein Kinase C - deficiency
- Receptor-CD3 Complex, Antigen, T-Cell - metabolism
- T-Lymphocytes - cytology
- Transcription Factors - genetics
- Transcriptional Activation - genetics
- Find related publications in this database (Keywords)
- T lymphocyte
- PKC theta
- TCR/CD3
- Ca2+ response
- NFAT