Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Ahammer, H; DeVaney, TT; Hartbauer, M; Tritthart, HA.
Cross-talk reduction in confocal images of dual fluorescence labelled cell spheroids.
Micron. 1999; 30(4):309-317
Doi: 10.1016/S0968-4328(99)00029-3
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Ahammer Helmut
- Co-Autor*innen der Med Uni Graz
- DeVaney Trevor
- Tritthart Helmut
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- Abstract:
- Dual fluorescence labelling is an advanced method to separate two individual specimens in a biological system using confocal microscopy. An inherent problem of this method is fluorescence channel cross-talk, which causes problems for the exact spatial determination and separation of the specimens. Using a parallel fluorescence detection and an image processing technique, based on an image subtraction method, we have developed a very straight forward method for correcting the dual channel fluorescence images. We successfully applied this method to a 3-dimensional cancer spheroid invasion assay and controlled the cross-talk compensation efficiency by a quality parameter.
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- Confocal Microscopy
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