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Selected Publication:
Seinost, G; Golde, WT; Berger, BW; Dunn, JJ; Qiu, D; Dunkin, DS; Dykhuizen, DE; Luft, BJ; Dattwyler, RJ.
Infection with multiple strains of Borrelia burgdorferi sensu stricto in patients with Lyme disease.
Arch Dermatol. 1999; 135(11): 1329-1333.
Doi: 10.1001/archderm.135.11.1329
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- Leading authors Med Uni Graz
- Seinost Gerald
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- Abstract:
- OBJECTIVE: To assess human skin biopsy specimens from erythema migrans lesions for the presence of infection with multiple strains of the Lyme disease spirochete, Borrelia burgdorferi. DESIGN: Skin biopsy specimens were obtained prospectively from patients with erythema migrans. To determine allelic differences and strain identification of B burgdorferi, the biopsy specimens were analyzed by cold single-strand conformation polymorphism of an amplified fragment of the outer surface protein C (ospC) gene. Further single-strand conformation polymorphism patterns of amplified ospC genes from culture isolates were compared with polymerase chain reaction products obtained directly from erythema migrans biopsy specimens. SETTING: A private dermatology office and a university medical center outpatient department. PATIENTS: Sixteen patients presenting with erythema migrans. RESULTS: Two of the 16 patients in this cohort were infected with 2 B burgdorferi sensu stricto strains, as evidenced by 2 ospC alleles in their skin biopsy results. CONCLUSION: This is the first documented description of the existence of more than a single strain of B burgdorferi sensu stricto in a human specimen.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Alleles -
- Antigens, Bacterial -
- Bacterial Outer Membrane Proteins - genetics
- Biopsy - genetics
- Borrelia burgdorferi - genetics
- Borrelia burgdorferi Group - classification
- Cohort Studies - classification
- Comparative Study - classification
- DNA, Bacterial - analysis
- Erythema Chronicum Migrans - microbiology
- Gene Expression Regulation, Bacterial - microbiology
- Humans - microbiology
- Lyme Disease - microbiology
- Polymerase Chain Reaction - microbiology
- Polymorphism, Single-Stranded Conformational - microbiology
- Prospective Studies - microbiology
- Research Support, Non-U.S. Gov't - microbiology
- Sequence Analysis, DNA - microbiology
- Skin - microbiology