Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Dandachi, N; Hauser-Kronberger, C; Moré, E; Wiesener, B; Hacker, GW; Dietze, O; Wirl, G.
Co-expression of tenascin-C and vimentin in human breast cancer cells indicates phenotypic transdifferentiation during tumour progression: correlation with histopathological parameters, hormone receptors, and oncoproteins.
J Pathol. 2001; 193(2):181-189
Doi: 10.1002/1096-9896(2000)9999:9999<::AID-PATH752>3.0.CO;2-V
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Dandachi Nadia
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- Abstract:
- Loss of epithelial morphology and the acquisition of mesenchymal characteristics are typical for carcinoma cells in tumour progression. In human breast carcinomas, up-regulation of tenascin-C (TN-C) and vimentin (Vim) is frequently observed in cancer cells and correlates with increased malignancy. Thus, it is possible that TN-C is co-expressed with Vim, representing cancer cells that have undergone epithelial-mesenchymal transition (EMT). This study examined 128 breast carcinomas using immunohistochemical techniques to demonstrate that mammary cancer cells are a prominent source of both TN-C and Vim. Statistical analysis revealed a significant association between TN-C and Vim expression in cancer cells. TN-C expression also correlated positively with overexpression of c-erbB-2 oncoprotein and down-regulation of oestrogen receptors (ERs). Eleven human mammary cancer cell lines and two 'normal' cell lines were examined by western blotting and immunohistochemistry. Co-expression of TN-C and Vim was detected in the carcinosarcoma cell line HS 578T, SK-BR-3 (B), fibroblast-like MDA-MB-231 cells, and the myoepithelial cell line HBL 100. These findings suggest that TN-C and Vim, when co-expressed in mammary carcinoma cells, represent regulator genes likely to be involved in EMT during mammary carcinogenesis.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Blotting, Western -
- Breast Neoplasms - metabolism
- Carcinoma, Ductal, Breast - metabolism
- Carcinoma, Lobular - metabolism
- Down-Regulation - metabolism
- Female - metabolism
- Humans - metabolism
- Middle Aged - metabolism
- Oncogene Proteins v-erbB - metabolism
- Phenotype - metabolism
- Receptors, Estrogen - metabolism
- Research Support, Non-U.S. Gov't - metabolism
- Tenascin - metabolism
- Tumor Cells, Cultured - metabolism
- Vimentin - metabolism
- Find related publications in this database (Keywords)
- human breast carcinoma
- tenascin-C
- vimentin
- epithelial-mesenchymal transition
- clinical parameters
- hormone receptors
- c-erbB-2