Medizinische Universität Graz - Research portal
Selected Publication:
Knauer, A; Wintersteiger, R; Markl, R; Sametz, W; Juan, H.
Derivatization of Carboxylic-Acids with Fluorescent Reagents
JPC-J PLANAR CHROMAT 1999 12: 211-214.
Web of Science
- Co-authors Med Uni Graz
- Juan Heinz
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- Abstract:
- This paper reports on the derivatization of carboxylic acids with fluorescent reagents after separation by thin-layer chromatography on normal- (silica gel) and reversed-phase plates. Two of the most promising reagents were compared to determine which resulted in the highest sensitivity. Derivatization with 4-bromomethyl-7-methoxycoumarin (BrMmC) was performed in the presence of 18-crown-6 as catalyst. The highly fluorescent esters could be detected by fluorodensitometry at an excitation wavelength of 360 nm with a cut off filter for emission. To improve the results the thin-layer plates were spray-coated with a solution of 10% poly(ethylene glycol) 4000 in chloroform. Derivatization by esterification with panacyl bromide (p-(9-anthroyloxy)phenacyl bromide, PAN), with the same crown ether as catalyst, was also investigated. Because this reagent furnished more intensely fluorescent products, the reaction conditions were further optimized by use of palmitic, propionic, and arachidonic acids as model substances. The limit of detection was in the lower picomole range.
- Find related publications in this database (Keywords)
- Carboxylic Acids
- Derivatization by Fluorescent Agents
- Brmmc
- Pan
- Fluorodensitometric Determination