Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Ardjomand, N; Komericki, P; Radner, H; Aigner, R; Reich, ME.
Corneal Langerhans cells. Behavior during storage in organ culture
OPHTHALMOLOGE. 1997; 94(10): 703-706.
Doi: 10.1007/s003470050188
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Ardjomand Navid
- Co-Autor*innen der Med Uni Graz
- Aigner Reingard
- Komericki Peter
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- Abstract:
- BACKGROUND: Corneal HLA-DR antigens are going to be lost during organ culture storage. This study investigated if this phenomenon is based on down-regulation of the HLA-DR antigen, or on a loss of the HLA-DR-positive corneal Langerhans cells (LCs). MATERIAL AND METHODS: Corneal LCs were stained in situ by the method of fluorescence-associated immunohistochemistry, and the organ culture mediums underwent flow cytometric analysis for HLA-DR-positive corneal LC at the end of the storage period. RESULTS: All stored corneas were negative for HLA-DR after 14 days and HLA-DR antigens could be detected in culture medium at the end of the storage time. CONCLUSION: Flow cytometry showed that organ culture storage leads to loss of HLA-DR-positive cells and not only to a loss of antigen presentation.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigen Presentation - immunology
- Cornea - immunology
- Corneal Transplantation - immunology
- Cryopreservation - immunology
- HLA-DR Antigens - analysis
- Humans - analysis
- Immune Tolerance - immunology
- Langerhans Cells - immunology
- Microscopy, Fluorescence - immunology
- Organ Preservation - immunology
- Time Factors - immunology
- Find related publications in this database (Keywords)
- Flow Cytometry
- HLA-Dr
- Langerhans Cells
- Organ Culture