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Stefulj, J; Cicin-Sain, L; Schauenstein, K; Jernej, B.
Serotonin and immune response: effect of the amine on in vitro proliferation of rat lymphocytes.
Neuroimmunomodulation. 2001; 9(2):103-108 Doi: 10.1159/000049013
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Co-authors Med Uni Graz: Schauenstein Konrad
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Abstract:: OBJECTIVE: The effect of serotonin (5-hydroxytryptamine; 5HT) on the in vitro proliferation of mitogen-stimulated lymphocytes was studied in primary cultures of rat spleen cells. METHODS: 5HT was added to the cultures 1 h prior to the mitogen, at final concentrations from 10(-13) up to 10(-2) M. T and B cell mitogens (concanavalin A, pokeweed mitogen and lipopolysaccharide) were used at suboptimal and optimal concentrations. The cell proliferation was measured 24-72 h after the addition of mitogen. The effect of each 5HT concentration was studied on a group of 6-12 animals and was expressed as a percentage of the control values obtained with mitogen alone. RESULTS: No significant effect of 5HT at concentrations from 10(-13) to 10(-5) M was found. At concentrations of > or =10(-4) M, a regular dose-dependent inhibition of the lymphocyte proliferation appeared, the concentration producing the half-maximal effect being 6 x 10(-4) M. The observed suppression was not due to 5HT cytotoxicity toward spleen cells. CONCLUSION: With the experimental system used, we failed to confirm an immunostimulatory effect of 5HT in the range of concentrations of its receptor sensitivities or lower, but found a clear-cut immunoinhibitory effect at higher concentrations.
Find related publications in this database (using NLM MeSH Indexing): Animals -; B-Lymphocytes - cytology; Cell Division - drug effects; Cells, Cultured - drug effects; Concanavalin A - pharmacology; Male - pharmacology; Mitogens - pharmacology; Neuroimmunomodulation - immunology; Rats - immunology; Rats, Sprague-Dawley - immunology; Serotonin - immunology; T-Lymphocytes - cytology
Find related publications in this database (Keywords): serotonin; lymphocyte; proliferation; mitogen