Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Thonhofer, R; Maercker, C; Popper, HH.
Expression of sarcoidosis related genes in lung lavage cells.
Sarcoidosis Vasc Diffuse Lung Dis. 2002; 19(1):59-65
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PubMed
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- Führende Autor*innen der Med Uni Graz
- Popper Helmuth
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- Abstract:
- BACKGROUND: Sarcoidosis is a systemic granulomatous multiorgan disease of unknown cause. Some facts point to a genetically determined predisposition, like associations with certain Major Histocompatibility Antigens Class I- and II-types (MHC). Mycobacterial DNA has been found in about 30% of sarcoidosis patients within the granulomas, which has been interpreted as a probable trigger mechanism. METHODS: Cells from bronchoalveolar lavage (BAL) of sarcoidosis patients were stimulated with dead Mycobacterium avium and gene expression was studied after six hours. Gene expression was profiled by hybridization to PCR filters (Human UniGene Set RZPD-1). To avoid the detection of effects related to secondary and tertiary inflammatory messages, we compared gene expression in sarcoidosis with that of tuberculosis and extrinsic allergic alveolitis. After normalization genes up- or downregulated for a factor of at least 1.8 were further analyzed. Genes differentially expressed in sarcoidosis as well as in tuberculosis or EAA, respectively, were subtracted. RESULTS AND CONCLUSIONS: Four different genes were exclusively upregulated in sarcoidosis: fatty acid binding protein 4 (FABP4), B-MYB, and two EST's. FABP4 has already been described in some autoimmune diseases, but its function in sarcoidosis is unknown. B-MYB is a potent growth factor for haematopoietic cells including lymphocytes. It might be one of the central genes upregulated in sarcoidosis and by that inducing clonal expansion of sarcoidosis-antigen primed T-helper-lymphocytes. Clonal selection of T-helper cells might be facilitated by c-Akt together with Tcl-1, both also upregulated in sarcoidosis. For the two ESTs neither function nor location is known.
- Find related publications in this database (using NLM MeSH Indexing)
- Alveolitis, Extrinsic Allergic - genetics
- Blotting, Northern - genetics
- Bronchoalveolar Lavage Fluid - cytology
- Carrier Proteins - genetics
- Fatty Acid-Binding Proteins - genetics
- Fatty Acids - genetics
- Gene Expression - genetics
- Gene Expression Regulation, Bacterial - genetics
- Humans - genetics
- Lung - ultrastructure
- Mycobacterium avium - genetics
- Neoplasm Proteins - genetics
- Oligonucleotide Array Sequence Analysis - genetics
- Oncogene Proteins - genetics
- Protein-Serine-Threonine Kinases - genetics
- Protein-Tyrosine Kinases - genetics
- Proto-Oncogene Proteins - genetics
- Proto-Oncogene Proteins c-akt - genetics
- Sarcoidosis - genetics
- Tuberculosis, Pulmonary - genetics
- Tumor Suppressor Proteins - genetics
- Up-Regulation - genetics
- Find related publications in this database (Keywords)
- sarcoidosis
- differential gene expression
- lavage cells
- mycobacteria-stimulated