Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schicho, R; Florian, W; Liebmann, I; Holzer, P; Lippe, IT.
Increased expression of TRPV1 receptor in dorsal root ganglia by acid insult of the rat gastric mucosa.
EUR J NEUROSCI. 2004; 19(7): 1811-1818.
Doi: 10.1111/j.1460-9568.2004.03290.x
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PubMed
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- Führende Autor*innen der Med Uni Graz
- Schicho Rudolf
- Co-Autor*innen der Med Uni Graz
- Holzer Peter
- Lippe Irmgard Theresia
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- Abstract:
- It is still unknown which receptors of peripheral sensory pathways encode and integrate an acid-induced nociceptive event in the gastric mucosa. The transient receptor potential vanilloid receptor 1 (TRPV1) and the acid-sensing ion channel 3 (ASIC3) are two nociception-related receptors. Here we investigated (i) to what extent these receptors are distributed in stomach-innervating neurons of dorsal root and nodose ganglia, using immunohistochemistry and retrograde tracing, and (ii) whether their expression is altered in response to a noxious acid challenge of the stomach. We also explored the presence of TRPV1 in the gastric enteric nervous system because of its possible expression by intrinsic sensory neurons. Most stomach-innervating neurons in nodose ganglia were immunoreactive for TRPV1 (80%) and ASIC3 (75%), these results being similar in the dorsal root ganglia (71 and 82%). RT-PCR and Western blotting were performed up to 6 h after oral application of 0.5 m HCl to conscious rats. TRPV1 protein was increased in dorsal root but not in nodose ganglia whereas TRPV1 and ASIC3 mRNAs remained unchanged. TRPV1 mRNA was detected in longitudinal muscle-myenteric plexus preparations of control stomachs and was not altered by the acid challenge. Combined vagotomy and ganglionectomy abolished expression of TRPV1, indicating that it may derive from an extrinsic source. In summary, noxious acid challenge of the stomach increased TRPV1 protein in spinal but not vagal or intrinsic sensory afferents. The TRPV1 receptor may be a key molecule in the transduction of acid-induced nociception of the gastric mucosa and a mediator of visceral hypersensitivity.
- Find related publications in this database (using NLM MeSH Indexing)
- Acids - pharmacology
- Animals -
- Benzofurans - metabolism
- Blotting, Western - methods
- Cell Count - methods
- Female -
- Ganglia, Spinal - cytology Ganglia, Spinal - drug effects Ganglia, Spinal - metabolism
- Ganglionectomy - methods
- Gastric Mucosa - cytology Gastric Mucosa - drug effects
- Immunohistochemistry - methods
- Membrane Proteins - genetics Membrane Proteins - metabolism
- Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism
- Neurons, Afferent - drug effects Neurons, Afferent - metabolism
- Nodose Ganglion - cytology Nodose Ganglion - drug effects Nodose Ganglion - metabolism
- RNA, Messenger - biosynthesis
- Rats -
- Rats, Sprague-Dawley -
- Receptors, Drug - genetics Receptors, Drug - metabolism
- Reverse Transcriptase Polymerase Chain Reaction - methods
- Sodium Channels - genetics Sodium Channels - metabolism
- Vagotomy - methods
- Find related publications in this database (Keywords)
- receptor up-regulation
- sensitization
- nociception
- mucosal injury