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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Koidl, C; Michael, B; Berg, J; Stöcher, M; Mühlbauer, G; Grisold, AJ; Marth, E; Kessler, HH.
Detection of transfusion transmitted virus DNA by real-time PCR.
J Clin Virol. 2004; 29(4):277-281
Doi: 10.1016/S1386-6532(03)00168-9
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Koidl Christoph
- Co-authors Med Uni Graz
- Berg Jörg
- Bozic Michael
- Grisold Andrea
- Kessler Harald
- Marth Egon
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- Abstract:
- Little is known about the pathogenic role and the endemic situation of transfusion transmitted virus (TTV). In this study, a molecular assay for detection of TTV based on automated nucleic acid extraction and real-time PCR was developed and evaluated. The new assay includes an internal control. After optimization of the molecular assay, 103 clinical samples were studied retrospectively. All sera had been tested for anti-HCV and anti-HIV-1 antibodies earlier. The amplification efficiency was found to be 102%. When clinical specimens were tested, 79 of 103 serum samples were found to be positive for TTV. There was no significant difference between various groups of patients. The internal control was detected in all negative and weak positive samples. This molecular assay proved to be suitable for routine detection of TTV in clinical samples. Moreover, a relative statement on the TTV serum load can be done.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Child -
- Child, Preschool -
- DNA Virus Infections - diagnosis DNA Virus Infections - virology
- DNA, Viral - blood
- Female -
- Humans -
- Male -
- Middle Aged -
- Polymerase Chain Reaction - methods
- Reference Standards -
- Renal Dialysis -
- Sensitivity and Specificity -
- Torque teno virus - genetics Torque teno virus - isolation & purification
- Viral Load -
- Viremia -
- Find related publications in this database (Keywords)
- TTV
- LightCycler
- real-time PCR
- automated extraction
- internal control