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Selected Publication:
Koeppel, TA; Trauner, M; Mennone, A; Arrese, M; Rios-Velez, L; Boyer, JL.
Role of glutathione in hepatic bile formation during reperfusion after cold ischemia of the rat liver.
J Hepatol. 1998; 28(5):812-819
Doi: 10.1016/S0168-8278(98)80231-6
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- Co-authors Med Uni Graz
- Trauner Michael
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- Abstract:
- Background/Aims: Liver reperfusion following cold ischemia is frequently associated with diminished bile flow in patients undergoing liver transplantation, Glutathione is st major determinant of bile-acid independent bile flow, and the effects of cold ischemia on biliary glutathione excretion are unknown. Methods: We examined the effects of cold ischemia (University of Wisconsin solution (4 degrees C), 24 h) with subsequent reperfusion (100 min) on biliary glutathione excretion in a recirculating system, Since glutathione might represent an important antioxidant within the biliary tract and oxidative stress inn the biliary tract during reperfusion could contribute to the pathogenesis of bile duct injury after liver transplantation, we also assessed bile duct morphology in reperfused livers of mutant TR--rats, in whom biliary excretion of glutathione is already impaired, Results: Hepatic bile formation was diminished in reperfused Wistar Pat livers after cold ischemia. Biliary glutathione concentrations and output were significantly decreased and correlated with postischemic changes ist bile secretion. An increased biliary oxidized glutathione/glutathione ratio, indicating oxidative stress, was detected only immediately after the onset of reperfusion. Basal bile flow rates in TR--rat livers which were already markedly reduced in control-perfused livers, decreased further during the early but not the later reperfusion period. Reperfusion of both Wistar and TR--rat livers was not associated with electron microscopic evidence of bile duct damage. Conclusions: We conclude that impaired biliary excretion of glutathione contributes to decreased bile flow after cold ischemia, The absence of biliary glutathione does not appear to promote ultrastructural evidence of bile duct injury during reperfusion in the isolated perfused rat liver.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenosine -
- Allopurinol -
- Animals -
- Bile - secretion
- Bile Ducts - physiology Bile Ducts - ultrastructure
- Cold Temperature -
- Glutathione - physiology Glutathione - secretion
- Insulin -
- Ischemia - physiopathology
- Liver - blood supply Liver - physiology
- Male -
- Organ Preservation -
- Organ Preservation Solutions -
- Raffinose -
- Rats -
- Rats, Wistar -
- Regression Analysis -
- Reperfusion -
- Time Factors -
- Find related publications in this database (Keywords)
- bile duct injury
- bile secretion
- glutathione
- hepatic preservation/reperfusion injury