Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Leis, HJ; Fauler, G; Windischhofer, W.
Quantitative analysis of memantine in human plasma by gas chromatography/negative ion chemical ionization/mass spectrometry.
J MASS SPECTROMETRY 2002 37: 477-480.
Doi: 10.1002/jms.303
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Leis Hans-Joerg
- Co-Autor*innen der Med Uni Graz
- Fauler Günter
- Windischhofer Werner
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- Abstract:
- A sensitive and specific method for the determination of memantine in human plasma is presented. Memantine was extracted from plasma and derivatized to the pentafluorobenzoyl derivative in a one-step procedure avoiding any sample concentration steps. Amantadine was used as an internal standard. The compounds were measured by gas chromatography/negative ion chemical ionization mass spectrometry without any further processing. Using this detection mode, the fragment ions at m/z 353 and 325 were obtained at high relative abundance. Calibration graphs were linear over the range 0.117-30 ng ml(-1). At the limit of quantification (LOQ), the inter-assay precision was 2.00% and the intra-assay variability was 3.22%. The accuracy at the LOQ showed deviations of -1.42% (intra-assay) and -2.47% (inter-assay). The method is rugged, rapid and robust and was applied to the batch determination of memantine during pharmacokinetic profiling of the drug.
- Find related publications in this database (using NLM MeSH Indexing)
- Amantadine - blood
- Antiparkinson Agents - blood
- Biological Availability - blood
- Blood Preservation - blood
- Calibration - blood
- Cryopreservation - blood
- Humans - blood
- Mass Fragmentography - methods
- Memantine - blood
- Reference Standards - blood
- Reproducibility of Results - blood
- Sensitivity and Specificity - blood
- Specimen Handling - blood
- Spectrometry, Mass, Secondary Ion - methods
- Find related publications in this database (Keywords)
- memantine
- gas chromatography/negative ion chemical ionization mass spectrometry
- human plasma