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Selected Publication:
Gallová, J; Abuja, PM; Pregetter, M; Laggner, P; Prassl, R.
Site-specific effect of radical scavengers on the resistance of low density lipoprotein to copper-mediated oxidative stress: influence of alpha-tocopherol and temperature.
CHEM PHYS LIPIDS 1998 92: 139-149.
Doi: 10.1016/S0009-3084(98)00017-6
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- Leading authors Med Uni Graz
- Prassl Ruth
- Co-authors Med Uni Graz
- Abuja Peter Michael
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- Abstract:
- The radical scavenging capacity of active nitroxide spin label radicals located at different depths in the surface monolayer of native and alpha-tocopherol enriched low density lipoprotein (LDL) has been evaluated at early stages of copper-mediated lipid peroxidation. Spin labels induced a concentration-dependent prolongation in lag time and a pronounced decrease in the initial rate of conjugated diene (CD) formation. These effects strongly argue for a protective, antioxidative action of spin labels, which in turn become destroyed with the extent of oxidation by radical recombination reactions. The results revealed that the decrease in spectral intensity proceeds at a higher rate for nitroxide radicals located in a more hydrophobic environment. The loss in spin label activity is accompanied by simultaneous alpha-tocopherol consumption and progresses rather independently of initial alpha-tocopherol content. The data provided no evidence that spin labels either save alpha-tocopherol or compete with it for radicals. The authors, therefore, deduce that due to enhanced accessibility and mobility, spin labels located in the interior of LDL eliminate lipid-derived radicals, which otherwise would promote lipid peroxidation. Lowering of temperature clearly below the core-lipid phase transition temperature of LDL exerts a significant effect on the kinetics of copper-induced LDL oxidation, whereas the characteristics of the radical scavenging mechanisms of the spin label molecules located in the surrounding phospholipid monolayer are conserved. Taken together, the susceptibility of LDL to primary oxidative stress conditions was efficiently retarded by small amounts of radical scavengers. This effect was more pronounced for nitroxide radicals embedded deeper in the phospholipid monolayer and was rather independent of alpha-tocopherol enrichment.
- Find related publications in this database (using NLM MeSH Indexing)
- Copper - pharmacology
- Cyclic N-Oxides - pharmacology
- Electron Spin Resonance Spectroscopy - pharmacology
- Free Radical Scavengers - pharmacology
- Humans - pharmacology
- Kinetics - pharmacology
- Lipid Peroxidation - drug effects
- Lipoproteins, LDL - blood
- Oxidative Stress - drug effects
- Spin Labels - drug effects
- Temperature - drug effects
- Vitamin E - pharmacology
- Find related publications in this database (Keywords)
- copper-mediated oxidation
- conjugated diene formation
- spin labels
- EPR-spectroscopy