Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Albertini, R; De Luca, G; Passi, A; Moratti, R; Abuja, PM.
Chondroitin-4-sulfate protects high-density lipoprotein against copper-dependent oxidation.
ARCH BIOCHEM BIOPHYS 1999 365: 143-149.
Doi: 10.1006/abbi.1999.1150
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Abuja Peter Michael
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- We investigated the effect of chondroitinsulphate (CS), the major glycosaminoglycan of the arterial wall, on the oxidation of human high-density lipoprotein (HDL) by kinetic analysis. Chondroitin-4-sulfate (C4S) increased the lag time and reduced the maximum rate of HDL oxidation induced by Cu2+, as assessed by monitoring both conjugated diene formation and low-level chemiluminescence. On the contrary, chondroitin-6-sulfate (C6S) was ineffective. Dermatansulfate exhibited an inhibitory effect comparable to that of C4S. C4S protected also the protein moiety of HDL, as it reduced tryptophan destruction by lipid-oxidizing species and delayed the formation of fluorescent adducts between end products of lipid peroxidation and amino acid residues. Again, C6S was ineffective. C4S was able to bind Cu2+; this resulted in less Cu2+ available for HDL oxidation and likely represented the mechanism of the protective effect. Neither C4S nor C6S affected HDL oxidation by peroxyl radicals, indicating that free radical scavenging activity was not involved in the protective effect. These results suggest that C4S might prevent the oxidative modification of HDL in arterial wall, thus preserving its antiatherogenic potential for reverse cholesterol transport and, possibly, for clearance of oxidized lipids.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Cations, Divalent - pharmacology
- Chondroitin Sulfates - pharmacology
- Copper - pharmacology
- Dermatan Sulfate - pharmacology
- Female - pharmacology
- Free Radical Scavengers - pharmacology
- Humans - pharmacology
- Kinetics - pharmacology
- Lipid Peroxidation - drug effects
- Lipoproteins, HDL - metabolism
- Male - metabolism
- Peroxides - metabolism
- Find related publications in this database (Keywords)
- chondroitinsulfate
- glycosaminoglycans
- lipoproteins
- high-density lipoprotein
- lipid peroxidation