Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Waldhoer, M; Wise, A; Milligan, G; Freissmuth, M; Nanoff, C.
Kinetics of ternary complex formation with fusion proteins composed of the A(1)-adenosine receptor and G protein alpha-subunits.
J Biol Chem. 1999; 274(43):30571-30579
Doi: 10.1074/jbc.274.43.30571
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- Führende Autor*innen der Med Uni Graz
- Waldhoer Maria
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- Abstract:
- High affinity agonist binding to G protein-coupled receptors depends on the formation of a ternary complex between agonist, receptor, and G protein. This process is too slow to be accounted for by a simple diffusion-controlled mechanism. We have tested if the interaction between activated receptor and G protein is rate-limiting by fusing the coding sequence of the human A(1)-adenosine receptor to that of Galpha(i-1) (A(1)/Galpha(i-1)) and of Galpha(o) (A(1)/Galpha(o)). Fusion proteins of the expected molecular mass were detected following transfection of HEK293 cells. Ternary complex formation was monitored by determining the kinetics for binding of the high affinity agonist (-)-N(6)-3[(125)I](iodo-4-hydroxyphenylisopropyl)adenosine; these were similar in the wild-type receptor and the fusion proteins over the temperature range of 10 to 30 degrees C. Agonist dissociation may be limited by the stability of the ternary complex. This assumption was tested by creating fusion proteins in which the Cys(351) of Galpha(i-1) was replaced with glycine (A(1)/Galpha(i-1)C351G) or isoleucine (A(1)/Galpha(i-1)C351I) to lower the affinity of the receptor for the G protein. In these mutated fusion proteins, the dissociation rate of the ternary complex was accelerated; in contrast, the rate of the forward reaction was not affected. We therefore conclude that (i) receptor activation per se rather than its interaction with the G protein is rate-limiting in ternary complex formation; (ii) the stability of the ternary complex is determined by the dissociation rate of the G protein. These features provide for a kinetic proofreading mechanism that sustains the fidelity of receptor-G protein coupling.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line -
- Cell Membrane - metabolism
- GTP-Binding Protein alpha Subunits, Gs - chemistry GTP-Binding Protein alpha Subunits, Gs - metabolism
- Humans -
- Iodine Radioisotopes -
- Kidney -
- Kinetics -
- Phenylisopropyladenosine - analogs & derivatives Phenylisopropyladenosine - pharmacokinetics
- Radioligand Assay -
- Receptors, Purinergic P1 - chemistry Receptors, Purinergic P1 - metabolism
- Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism
- Transfection -