Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Zhong, XY; Holzgreve, W; Gebhardt, S; Hillermann, R; Tofa, KC; Gupta, AK; Huppertz, B; Hahn, S.
Minimal alteration in the ratio of circulatory fetal DNA to fetal corticotropin-releasing hormone mRNA level in preeclampsia.
FETAL DIAGN THER. 2006; 21: 246-249.
Doi: 10.1159/000091349
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Huppertz Berthold
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- Abstract:
- OBJECTIVES: We have recently observed that fetal DNA and fetal corticotropin-releasing hormone (CRH) mRNA are associated with in vitro generated syncytiotrophoblast-derived microparticles, and that the ratio of fetal DNA to mRNA (CRH) varied according to whether the particles were derived by predominantly apoptotic, apo-necrotic or necrotic pathways. Hence, we examined whether these ratios varied in maternal plasma samples taken from normotensive and preeclamptic pregnancies in vivo. METHODS: Maternal plasma samples were collected from 18 cases with preeclampsia and 29 normotensive term controls. Circulatory fetal CRH mRNA and DNA levels were quantified by real-time PCR and RT-PCR. RESULTS: Circulatory fetal mRNA and fetal DNA levels were significantly elevated in the preeclampsia study group when compared to normotensive controls. Alterations in the fetal mRNA to DNA ratio between the study and control groups were minimal, even when stratified into early (<34 weeks of gestation) and late (>34 weeks of gestation) onset preeclampsia. CONCLUSIONS: Our data suggest that although circulatory fetal DNA and mRNA levels are significantly elevated in preeclampsia, the ratios in maternal plasma are not dramatically altered.
- Find related publications in this database (using NLM MeSH Indexing)
- Corticotropin-Releasing Hormone - genetics
- DNA - blood
- Female - blood
- Fetal Blood - chemistry
- Gestational Age - chemistry
- Humans - chemistry
- Polymerase Chain Reaction - chemistry
- Pre-Eclampsia - blood
- Pregnancy - blood
- RNA, Messenger - blood
- Research Support, Non-U.S. Gov't - blood
- Reverse Transcriptase Polymerase Chain Reaction - blood
- Find related publications in this database (Keywords)
- circulatory fetal DNA and RNA
- maternal plasma
- preeclampsia