Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Baczyk, D; Dunk, C; Huppertz, B; Maxwell, C; Reister, F; Giannoulias, D; Kingdom, JC.
Bi-potential behaviour of cytotrophoblasts in first trimester chorionic villi.
Placenta. 2005; 27(4-5):367-374
Doi: 10.1016/j.placenta.2005.03.006
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- Co-Autor*innen der Med Uni Graz
- Huppertz Berthold
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- Abstract:
- Murine trophoblast stem (TS) cells express fibroblast growth factor receptor 2 (FGFR2) and are maintained in their proliferative state by fibroblast growth factor 4 (FGF4). We show in this report that in the first trimester human placenta FGFR2 expression is similarly found in a subset of villous cytotrophoblast and in proximal anchoring columns. Western analysis demonstrated declining FGFR2 protein expression as gestation advanced, suggesting a similar role for FGF in early human trophoblast proliferation. Mouse TS cell differentiation is known to occur along two distinct transcriptionally-regulated pathways; extravillous trophoblast (EVT) cells invade the uterine wall to promote maternal blood flow whilst syncytiotrophoblast lines chorionic villi in the labyrinth. Similar differentiation steps occur in the human placenta though the fate of human trophoblast stem cells is presently unknown. To investigate the mechanisms underlying human cytotrophoblast differentiation we have developed a novel cultured floating first trimester villous explant model in which denuded first trimester villi spontaneously regenerate syncytiotrophoblast following 48 h of culture. Addition of FGF4 and heparin inhibited syncytiotrophoblast regeneration in favor of forming clumps of cytotrophoblast. Proximal cells in these clumps were FGFR2 immuno-reactive and proliferative, intermediate parts expressed alpha5beta1-integrin, while the distal portion expressed HLA-G and the invasive integrin alpha1beta1 indicating differentiation to the EVT phenotype. In contrast, non-denuded villi exposed to FGF4 exhibited similar proliferation of the cytotrophoblast; however, these cells did not express any of the invasive EVT markers. We conclude that FGFR2-positive chorionic cytotrophoblasts exhibit bi-potential behaviour, being capable of forming either syncytiotrophoblast or EVT. We suggest bipotential trophoblast progenitor cells persist during first trimester human placental development.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Differentiation -
- Chorionic Villi - physiology
- Female -
- Fibroblast Growth Factor 4 - physiology
- Giant Cells - cytology
- Humans -
- Mice -
- Pregnancy -
- Pregnancy Trimester, First - physiology
- Receptor, Fibroblast Growth Factor, Type 2 - metabolism
- Trophoblasts - cytology
- Find related publications in this database (Keywords)
- cytotrophoblast differentiation
- human placental villi
- fibroblast growth factor 4 (FG4)
- fibroblast growth factor receptor 2 (FGFR2)
- villous explant culture
- syncytial denudation
- syncytiotrophoblast
- extravillous trophoblast