Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Khoschsorur, G; Erwa, W.
Liquid chromatographic method for simultaneous determination of mycophenolic acid and its phenol- and acylglucuronide metabolites in plasma.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004; 799(2):355-360
Doi: 10.1016/j.jchromb.2003.10.074
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Khoschsorur Gholamali
- Co-Autor*innen der Med Uni Graz
- Erwa Wolfgang
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- Abstract:
- A simple, sensitive and reproducible HPLC method is presented for the simultaneous determination of mycophenolic acid (MPA) and its metabolites phenolic MPA-glucuronide (MPAG) and acyl glucuronide (AcMPAG) in human plasma. Sample purification requires protein precipitation with 0.1 M phosphoric acid/acetonitrile in the presence of Epilan D as an internal standard (IS). Separation was performed by reversed-phase HPLC, using a Zorbax SB-C18 column, 32% acetonitrile and a 40 mM phosphoric acid buffer at pH 3.0 as mobile phase; column temperature was 50 degrees C, flow rate 1.4 ml/min, and measurement by UV detection was at 215 nm (run time 12 min). The method requires only 50 microl plasma. Detection limits were 0.1 microg/ml for MPA and AcMPAG, and 2.0 microg/ml for MPAG, respectively. Mean absolute recovery of all three analytes was >95%. This analytical method for the determination of MPA and its metabolites is a reliable and convenient procedure that meets the criteria for application in routine clinical drug monitoring and pharmacokinetic studies.
- Find related publications in this database (using NLM MeSH Indexing)
- Chromatography, High Pressure Liquid - methods
- Glucuronides - blood
- Humans -
- Mycophenolic Acid - blood
- Reference Standards -
- Reproducibility of Results -
- Sensitivity and Specificity -
- Find related publications in this database (Keywords)
- mycophenolic acid