Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Daxboeck, F; Dornbusch, HJ; Krause, R; Assadian, O; Wenisch, C.
Verification of false-positive blood culture results generated by the BACTEC 9000 series by eubacterial 16S rDNA and panfungal 18S rDNA directed polymerase chain reaction (PCR).
Diagn Microbiol Infect Dis. 2004; 48(1):1-3
Doi: 10.1016/j.diagmicrobio.2003.08.001
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PubMed
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- Co-Autor*innen der Med Uni Graz
- Dornbusch Hans Jürgen
- Krause Robert
- Wenisch Christoph
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- Abstract:
- A small but significant proportion of blood cultures processed by the BACTEC 9000 series systems is signaled positive, while subsequent Gram's stain and culture on solid media yield no pathogens. In this study, 15 "false-positive" vials (7 aerobes, 8 anaerobes) from 15 patients were investigated for the presence of bacteria and fungi by eubacterial 16S rDNA and panfungal 18S rDNA amplification, respectively. All samples turned out negative by both methods. Most patients (7) had neutropenia, which does not support the theory that high leukocyte counts enhance the generation of false-positive results. In conclusion, the results of this study indicate that false-negative results generated by the BACTEC 9000 series are inherent to the automated detection and not due to the growth of fastidious organisms.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Bacteria, Aerobic - classification
- Bacteria, Anaerobic - classification
- Bacterial Typing Techniques - classification
- Base Sequence - classification
- Blood - microbiology
- False Positive Reactions - microbiology
- Female - microbiology
- Fungi - classification
- Humans - classification
- Male - classification
- Middle Aged - classification
- Molecular Sequence Data - classification
- Polymerase Chain Reaction - methods
- RNA, Ribosomal, 16S - analysis
- RNA, Ribosomal, 18S - analysis
- Sampling Studies - analysis
- Sensitivity and Specificity - analysis
- Find related publications in this database (Keywords)
- BACTEC 9000 series
- eubacterial 165 rDNA PCR
- panfungal 185 rDNA PCR