Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Poláková, K; Bandzuchová, E; Hofmeister, V; Weiss, EH; Hutter, H; Russ, G.
Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients.
Neoplasma. 2003; 50(5):331-338
Web of Science
PubMed
FullText
- Co-Autor*innen der Med Uni Graz
- Hutter Heinz
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- Abstract:
- Expression of HLA-G on the surface of malignant hematopoietic cells isolated from leukemia patients was analyzed by flow cytometry using monoclonal antibodies (mAbs) recognizing both, intact HLA-G complex (87G, 01G and MEM-G9) as well as HLA-G free heavy chain (4H84, MEM-G/1 and MEM-G/2). Prerequisite of HLA-G detection by mAbs specific to free heavy chain was mild acid treatment, which dissociates intact HLA-G complex. All mAbs, with the exception of 4H84 mAb, did not indicate the presence of HLA-G antigen in leukemia cells. Positive staining with 4H84 mAb was detected in acid-treated cells isolated from 16 out of 30 patients. Intensity of staining increased after IFN-g pre-incubation in most cases. Immunoblot analyses and RT-PCR, however, failed to detect HLA-G antigen or HLA-G transcripts in cells that bind 4H84 mAb after acid-treatment. The binding of 4H84 mAb can be explained by the acid-induced cross-reactivity of this HLA-G specific mAb with classical HLA class I molecules [15]. The results described here further demonstrate that the HLA-G molecule is not expressed in freshly isolated human leukemia cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Antibodies, Monoclonal - immunology
- Antibody Specificity - immunology
- Bone Marrow Cells - immunology
- Child - immunology
- Child, Preschool - immunology
- Female - immunology
- HLA Antigens - immunology
- Hematopoietic Stem Cells - immunology
- Histocompatibility Antigens Class I - immunology
- Humans - immunology
- Leukemia - blood
- Lymphoma - blood
- Male - blood
- Middle Aged - blood
- Find related publications in this database (Keywords)
- HLA-G antigen
- human leukemia cells
- flow cytometry
- immunoblotting
- RT-PCR