Medizinische Universität Graz - Research portal
Selected Publication:
Zenzmaier, C; Kollroser, M; Gesslbauer, B; Jandrositz, A; Preisegger, KH; Kungl, AJ.
Preliminary 2-D chromatographic investigation of the human stem cell proteome.
Biochem Biophys Res Commun. 2003; 310(2):483-490
Doi: 10.1016%2Fj.bbrc.2003.09.036
Web of Science
PubMed
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FullText_MUG
- Co-authors Med Uni Graz
- Kollroser Manfred
- Preisegger Karl Heinz
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- Abstract:
- Stem cells represent a promising tool for the treatment of various hematopoietic diseases. In order to identify stem cell-specific proteins, the proteome of human stem cells from umbilical cord blood was explored for the first time. For this purpose, the crude lysate of 4 x 10(5) CD34+ cells was subjected to in solution trypsin digestion. The resulting peptides were then separated via cation exchange followed by reversed phase chromatography and analyzed by nanospray MS/MS. Database search revealed a total of 215 proteins which could be reliably identified. To obtain a more complete picture of the human stell cell proteome and to also access low abundant proteins, pooling of more than one CD34+ preparations seems necessary in order to increase the cell number and thus the protein content.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD34 - analysis
- Chromatography, High Pressure Liquid - analysis
- Female - analysis
- Fetal Blood - cytology
- Humans - cytology
- Proteome - isolation and purification
- Spectrometry, Mass, Electrospray Ionization - isolation and purification
- Stem Cells - chemistry
- Find related publications in this database (Keywords)
- stem cells
- 2-D liquid chromatography
- umbilical cord blood
- mass spectrometry