Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Regauer, S; Beham-Schmid, C.
Detailed analysis of the T-cell lymphocytic infiltrate in penile lichen sclerosus: an immunohistochemical and molecular investigation.
Histopathology. 2006; 48(6):730-735
Doi: 10.1111/j.1365-2559.2006.02406.x
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Regauer Sigrid
- Co-Autor*innen der Med Uni Graz
- Beham-Schmid Christine
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- AIM: To determine the extent of clonal outgrowth in the lymphocytic tissue infiltrate of lichen sclerosus (LS). The presence of T cells with a monoclonally rearranged T-cell receptor gamma-gene (TCRgamma) has been described in up to 50% of biopsies of vulvar and penile LS. MATERIAL AND RESULTS: We analysed 33 foreskin specimens with LS for the presence of clonal T cells by conventional polymerase chain reaction (PCR) analysis and with TCRgamma-PCR-based fluorescent fragment analysis. Eighteen of 33 patients revealed a band indicating a monoclonally rearranged TCRgamma on conventional PCR analysis. Subsequent TCRgamma-PCR-based fluorescent fragment analysis identified 8/18 patients with monoclonal T-cell DNA ranging from 1.4% to 23.1% of total T-cell DNA analysed and a size range from 56 to 72 base pairs. Four of 18 patients had an oligoclonal and 6/18 patients revealed a polyclonal banding pattern. The lymphocytic infiltrate contained low numbers of gammadelta T cells and cytotoxic T cells in comparable numbers to the low percentage of clonal TCRgamma DNA. CONCLUSIONS: The low percentage of clonal TCRgamma DNA argues against a systemic neoplastic disease, but rather for a local immune disorder. The target antigen of the clonal outgrowth is unknown, but an exaggerated antigen-dependent proliferation of T cells due to chronic local antigen exposure, probably an infectious antigen, is the most likely explanation.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Antigens, CD3 - analysis
- Antigens, CD4 - analysis
- Antigens, CD7 - analysis
- Antigens, CD8 - analysis
- Child - analysis
- Child, Preschool - analysis
- Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor - genetics
- Humans - genetics
- Immunohistochemistry - genetics
- Lichen Sclerosus et Atrophicus - immunology
- Male - immunology
- Middle Aged - immunology
- Penile Diseases - immunology
- Polymerase Chain Reaction - immunology
- T-Lymphocytes - chemistry
- Find related publications in this database (Keywords)
- antigen
- dermatosis
- immune dysregulation
- lymphoid infiltrate
- monoclonality