Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Hauer, AC; Riederer, M; Griessl, A; Rosegger, H; MacDonald, TT.
Cytokine production by cord blood mononuclear cells stimulated with cows milk proteins in vitro: interleukin-4 and transforming growth factor beta-secreting cells detected in the CD45RO T cell population in children of atopic mothers.
Clin Exp Allergy. 2003; 33(5):615-623
Doi: 10.1046%2Fj.1365-2222.2003.01646.x
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Hauer Almuthe
- Co-Autor*innen der Med Uni Graz
- Riederer Monika
- Rosegger Helfried
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- Abstract:
- BACKGROUND: Food antigens from the maternal circulation may sensitize fetal T cells in utero and be an important determinant in the development of food allergy. METHODS: Here we have examined the spontaneous and recall response to cow's milk proteins of cord blood mononuclear cells (CBMC) of newborn children, using single cell ELISPOT assays. RESULTS: In term newborns, confirming previous studies, the spontaneous cytokine response of CBMC is dominated by IL-4, IL-5, IL-10, and as shown here for the first time, TGF-beta. For TGF-beta only, the response of samples from infants of atopic mothers was significantly lower than samples from infants of non-atopic mothers. In vitro stimulation of CBMC with bovine serum albumin, casein and beta-lactoglobulin resulted in a significant increase of all cytokine-secreting cells, again dominated by T helper type 2 (Th2) cytokines. There was a clear tendency for samples from infants of atopic mothers to have lower Th2 responses than samples from infants of non-atopic mothers, which was particularly significant for both IL-4 and TGF-beta. Spontaneous cytokine secreting cells were virtually absent in cord blood from infants < 34 weeks gestation, as were cows milk protein-induced responses, although they were readily detectable in samples from infants aged > 34 weeks. To explore whether the cytokine secreting cells were in the naive CD4+ CD45RA population or memory CD4+ CD45RO T cells, these subsets were purified by positive and negative selection and tested for spontaneous and cows milk protein-induced cytokine responses. Strikingly, although the responses were small, the CD45RO+ cells from children of atopic mothers showed significant spontaneous and antigen-specific IL-4 and TGF-beta responses, whereas the same population from infants of non-atopic mothers showed virtually no response. In addition CD45RA+ cells from infants of mothers with maternal atopy showed decreased IL-4 and TGF-beta responses, especially the latter. CONCLUSIONS: The cows milk antigen-specific IL-4 and TGF-beta responses preferentially seen in the memory cell subset of infants with a maternal history of atopy strongly suggests Th2 skewing to dietary antigens in utero.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antigens, CD45 - analysis
- CD4-Positive T-Lymphocytes - immunology
- Cells, Cultured - immunology
- Cytokines - biosynthesis
- Female - biosynthesis
- Fetal Blood - immunology
- Humans - immunology
- Hypersensitivity, Immediate - immunology
- Infant, Newborn - immunology
- Infant, Premature - immunology
- Interleukin-4 - biosynthesis
- Male - biosynthesis
- Maternal-Fetal Exchange - immunology
- Milk Proteins - immunology
- Pregnancy - immunology
- Pregnancy Complications - immunology
- T-Lymphocyte Subsets - immunology
- Transforming Growth Factor beta - biosynthesis
- Find related publications in this database (Keywords)
- atopy
- ELISPOT
- interleukin-4
- memory T cells
- newborns
- transforming growth factor-beta