Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Regitnig, P; van Paasen, R; Tsybrovskyy, O.
How to improve microscopic images obtained with consumer-type digital cameras.
Histopathology. 2003; 42(5):503-508
Doi: 10.1046/j.1365-2559.2003.01604.x
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Regitnig Peter
- Co-Autor*innen der Med Uni Graz
- Tsybrovskyy Oleksiy
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- Abstract:
- AIM: Digital imaging is useful in conventional photography because it immediately provides images, and the image quality can be improved afterwards by the use of computer programs. The major disadvantages of consumer-type digital cameras mounted on microscopes are (i) unequal illumination through the image, and (ii) a coloured background. A computer program was specifically adapted and refined to improve images obtained with consumer-type digital cameras mounted on microscopes. METHODS AND RESULTS: An approach using a division operation between the specimen image and a background image leads to homogeneous illumination throughout the image, with automatically corrected brightness and white background. The correct colour spectrum is preserved by correction of the histogram. This approach was obtained from the freeware computer program 'Image Arithmetic'. In a test, three different consumer-type digital cameras (Sony, Nikon, Olympus) on different microscopes were used to obtain images of different types of histological specimens (cervical smear, bone marrow biopsy, and colonic biopsy). The computer program dramatically improved the quality of images obtained with all tested cameras. CONCLUSION: Using this approach, even low-cost digital cameras mounted on microscopes produce brilliant images with homogeneous illumination and a white background, the image quality being comparable with expensive cameras especially designed for microscopes.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans -
- Image Enhancement - methods
- Photomicrography - economics
- Signal Processing, Computer-Assisted - instrumentation
- Software - instrumentation
- Find related publications in this database (Keywords)
- digital microphotography
- background compensation
- image improvement
- processing
- arithmetical operation
- division