Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Stadlbauer, V; Schaffellner, S; Iberer, F; Lackner, C; Liegl, B; Zink, B; Kniepeiss, D; Tscheliessnigg, KH.
Occurance of apoptosis during ischemia in porcine pancreas islet cells.
Int J Artif Organs. 2003; 26(3):205-210
Doi: 10.1177/039139880302600305
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Stadlbauer-Köllner Vanessa
- Co-Autor*innen der Med Uni Graz
- Iberer Florian
- Kniepeiss Daniela
- Lackner Karoline
- Liegl-Atzwanger Bernadette
- Schaffellner Silvia
- Tscheliessnigg Karlheinz
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- Abstract:
- Purpose: Pancreas islet transplantation is a potential treatment of diabetes mellitus and porcine organs provide an easily available source of cells. Unfortunately quality and quantity of isolated islets are still not satisfactory. Apoptosis occurs in freshly isolated islets and plays a significant role in early graft loss. We evaluated the influence of four storage solutions on porcine pancreas islets.Method: After warm ischemia of 15-20 minutes 12 organs were stored in 4 cold preservation solutions: Histidine-Tryptophan-Ketoglutarate solution (HTK), Hank's buffered saline solution (HBSS), University of Wisconsin (UW) solution and Ringer-Lactate (R). After cold ischemia for 100 minutes, organs were fixed in 3% formalin. Apoptotic cells were counted on hematocylin-eosin stainings.Results: Most apoptotic cells were found in organs stored in R. Low numbers were found in the other groups. The difference between organs stored in R and organs stored in UW HTK, or HBSS was highly significant No significant difference could be found between UW, HTK and HBSS.Conclusion: Cold and warm ischemia of the pancreas seems to induce apoptosis in islet cells. Preservation solutions cause less apoptosis than electrolyte solution. No significant differences could be found among the preservation solutions.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Apoptosis - drug effects Apoptosis - physiology
- Female -
- Hypothermia, Induced - methods
- Ischemia - pathology Ischemia - physiopathology
- Islets of Langerhans - blood supply Islets of Langerhans - drug effects Islets of Langerhans - physiopathology
- Islets of Langerhans Transplantation -
- Male -
- Organ Preservation - methods
- Organ Preservation Solutions - therapeutic use
- Pancreas - blood supply Pancreas - drug effects Pancreas - physiopathology
- Swine -
- Find related publications in this database (Keywords)
- porcine pancreas
- apoptosis
- pancreas islet transplantation