Medizinische Universität Graz - Research portal
Selected Publication:
Selenko-Gebauer, N; Majdic, O; Szekeres, A; Höfler, G; Guthann, E; Korthäuer, U; Zlabinger, G; Steinberger, P; Pickl, WF; Stockinger, H; Knapp, W; Stöckl, J.
B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy.
J Immunol. 2003; 170(7):3637-3644
Doi: 10.4049/jimmunol.170.7.3637
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-authors Med Uni Graz
- Höfler Gerald
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- In an effort to identify immunoregulatory molecules on dendritic cells (DC), we generated and screened for mAbs capable of modulating the T cell stimulatory function of DC. A particularly interesting mAb was mAb DF272. It recognizes monocyte-derived DC, but not blood monocytes or lymphocytes, and has profound immunomodulatory effects on DC. Treatment of DC with intact IgG or Fab of mAb DF272 enhanced their T cell stimulatory capacity. This effect on DC was accompanied by neither an up-regulation of costimulatory molecules such as B7.1 (CD80), B7.2 (CD86), and MHC class II molecules nor by an induction of cytokine production, including IL-1, TNF-alpha, IL-10, and IL-12. Moreover, the well-established inhibitory function of IL-10-treated DC could be reverted with mAb DF272. Even T cells, anergized because of stimulation with IL-10-treated DC, could be reactivated and induced to proliferate upon stimulation with mAb DF272-treated DC. Furthermore, mAb DF272-treated DC favored the induction of a type-1 cytokine response in T cells and inhibited IL-10 production. By using a retrovirus-based cDNA expression library generated from DC, we cloned and sequenced the mAb DF272-defined cell surface receptor and could demonstrate that it is identical with B7-H1 (programmed death-1 ligand), a recently identified new member of the B7 family of costimulatory molecules. Our results thus demonstrate that the mAb DF272-defined surface molecule B7-H1 represents a unique receptor structure on DC that might play a role in the induction and maintenance of T cell anergy.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antibodies, Blocking - metabolism
- Antibodies, Monoclonal - metabolism
- Antigens, CD - metabolism
- Antigens, CD80 - biosynthesis
- Apoptosis - immunology
- Binding Sites, Antibody - immunology
- Blood Proteins - biosynthesis
- Cells, Cultured - biosynthesis
- Clonal Anergy - immunology
- Coculture Techniques - immunology
- Cytokines - biosynthesis
- Dendritic Cells - immunology
- Epithelial Cells - immunology
- Humans - immunology
- Immunosuppressive Agents - antagonists and inhibitors
- Interleukin-10 - antagonists and inhibitors
- Ligands - antagonists and inhibitors
- Membrane Glycoproteins - antagonists and inhibitors
- Mice - antagonists and inhibitors
- Organ Specificity - immunology
- Peptides - immunology
- T-Lymphocytes - cytology
- Th1 Cells - immunology