Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Leis, HJ; Rechberger, GN; Fauler, G; Windischhofer, W.
Enantioselective trace analysis of amphetamine in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry.
Rapid Commun Mass Spectrom. 2003; 17(6):569-575
Doi: 10.1002/rcm.949
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Leis Hans-Joerg
- Co-Autor*innen der Med Uni Graz
- Fauler Günter
- Windischhofer Werner
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- Abstract:
- A method for the quantitative enantioselective analysis of amphetamine in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry (GC/NICI-MS) is presented. Five-fold deuterated analogues of both enantiomers were used as internal standard. Plasma sample preparation was performed by a rapid liquid-liquid extraction using n-hexane. Derivatization with (S)-(-)-N-(heptafluorobutyryl)prolyl chloride was accomplished directly in the n-hexane extract to avoid loss of amphetamine during sample concentration. The method was validated in the expected concentration range of 0.006 for a pharmacokinetic study. Calibration curves were linear within a range 0.006-50 ng/mL plasma. Precision and accuracy were acceptable over the entire calibration range. Baseline separation of the enantiomers was easily achieved on a 15-m nonchiral apolar column. The method is simple and robust, and has been applied to the batch analysis of amphetamine enantiomers.
- Find related publications in this database (using NLM MeSH Indexing)
- Amphetamine - blood
- Calibration - blood
- Chromatography, Gas - methods
- Drug Stability - methods
- Hexanes - methods
- Humans - methods
- Mass Spectrometry - methods
- Reproducibility of Results - methods
- Sensitivity and Specificity - methods
- Stereoisomerism - methods