Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wadsack, C; Hirschmugl, B; Hammer, A; Levak-Frank, S; Kozarsky, KF; Sattler, W; Malle, E.
Scavenger receptor class B, type I on non-malignant and malignant human epithelial cells mediates cholesteryl ester-uptake from high density lipoproteins.
Int J Biochem Cell Biol. 2003; 35(4):441-454
Doi: 10.1016/S1357-2725(02)00272-8
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- Führende Autor*innen der Med Uni Graz
- Malle Ernst
- Wadsack Christian
- Co-Autor*innen der Med Uni Graz
- Hammer Astrid
- Hirschmugl Birgit
- Levak Sanja
- Sattler Wolfgang
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- Abstract:
- Hepatoma cell lines serve as a suitable model to study hepatic clearance of lipoprotein-associated cholesteryl esters (CEs). The present study aimed at investigating holoparticle-association of and selective CE-uptake from human high density lipoprotein subclass 3 (HDL3) by non-malignant adult (Chang-liver) and non-malignant fetal (WRL-68) epithelial cell lines as well as a hepatocellular carcinoma (HUH-7) cell line. Binding properties of 125I-HDL3 at 4 and 37 degrees C were similar for all three cell lines while degradation rates were highest for Chang-liver cells. Calculating the selective uptake of HDL3-associated CEs as the difference between [3H]CE- and 125I-HDL3 cell-association revealed that the selective lipid uptake and holoparticle-association was similar in Chang-liver while in WRL-68 and HUH-7 cells pronounced capacity for lipid tracer uptake in excess of holoparticle uptake was measured. Using RT-PCR, Northern and Western blot analysis, as well as immunocytochemical technique pronounced expression of scavenger receptor class B, type I (SR-BI) but not SR-BII (a splice variant of SR-BI less efficient for selective CE-uptake than SR-BI) could be identified in HUH-7 and WRL-68 cells. A polyclonal antiserum raised against SR-BI significantly decreased cell-association of [3H]CE-HDL3 in HUH-7 and WRL-68. The present findings suggest that the capacity for selective cholesteryl ester-uptake from high density lipoprotein by malignant and normal epithelial cells from the liver depends on expression of the scavenger receptor class B, type I.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antigens, CD36 - metabolism
- CHO Cells - metabolism
- Carcinoma, Hepatocellular - metabolism
- Cholesterol Esters - metabolism
- Cricetinae - metabolism
- Epithelial Cells - metabolism
- Hepatocytes - metabolism
- Humans - metabolism
- Lipoproteins, HDL - metabolism
- Liver Neoplasms - metabolism
- Lysosome-Associated Membrane Glycoproteins - metabolism
- Membrane Proteins - metabolism
- Receptors, Lipoprotein - metabolism
- Scavenger Receptors, Class B - metabolism
- Sialoglycoproteins - metabolism
- Tumor Cells, Cultured - metabolism
- Find related publications in this database (Keywords)
- hepatoma cells
- HDL particle
- SR-BI
- holoparticle uptake
- Chang-liver
- HUH-7
- WRL-68