Medizinische Universität Graz - Research portal
Selected Publication:
Schicho, R; Schemann, M; Pabst, MA; Holzer, P; Lippe, IT.
Capsaicin-sensitive extrinsic afferents are involved in acid-induced activation of distinct myenteric neurons in the rat stomach.
Neurogastroenterol Motil. 2003; 15(1):33-44
Doi: 10.1046%2Fj.1365-2982.2003.00384.x
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Schicho Rudolf
- Co-authors Med Uni Graz
- Holzer Peter
- Lippe Irmgard Theresia
- Pabst Maria-Anna
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- Abstract:
- Challenge of the rat gastric mucosa with 0.5 mol L(-1) HCl activates nitrergic neurons in the myenteric plexus as visualized by c-Fos immunohistochemistry. In the present study, we characterized the activated neurons more extensively by their chemical coding and investigated whether a neural pathway that involves capsaicin-sensitive extrinsic afferents and/or cholinergic neurons transmitting via nicotinic receptors contributes to the activation of myenteric neurons. In multiple labelling experiments, c-Fos was examined for co-localization with nitric oxide synthase (NOS), vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), enkephalin (ENK), gastrin-releasing peptide (GRP), substance P (SP), calbindin D-28k (CALB) and neurofilament 145 (NF 145). All c-Fos-positive neurons were immunoreactive for NOS, VIP, NPY and NF 145, but not for SP, ENK, GRP and CALB. Nerve fibres co-expressing NOS, VIP and NPY were predominantly found in the external muscle layer and in the muscularis mucosae but rarely in the mucosa. Pre-treatment with capsaicin or hexamethonium or a combination of both pre-treatments reduced HCl-induced c-Fos expression by 54, 66 and 63%, respectively. Acid challenge of the stomach, therefore, leads to activation of presumably inhibitory motor neurons responsible for muscle relaxation. Activation of these neurons is partly mediated by capsaicin-sensitive afferents and involves ganglionic transmission via nicotinic receptors.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Capsaicin - pharmacology
- Cells, Cultured -
- Female -
- Ganglionic Blockers - pharmacology
- Gastric Mucosa - innervation Gastric Mucosa - metabolism
- Genes, fos - drug effects Genes, fos - physiology
- Hexamethonium - pharmacology
- Hydrochloric Acid - pharmacology
- Immunohistochemistry -
- Microscopy, Confocal -
- Myenteric Plexus - cytology Myenteric Plexus - drug effects Myenteric Plexus - physiology
- Neural Pathways - cytology Neural Pathways - drug effects
- Neurons, Afferent - drug effects Neurons, Afferent - physiology
- Rats -
- Rats, Sprague-Dawley -
- Find related publications in this database (Keywords)
- c-Fos
- capsaicin
- mucosal acid challenge
- multiple labelling
- neurochemical coding