Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wadsack, C; Hammer, A; Levak-Frank, S; Desoye, G; Kozarsky, KF; Hirschmugl, B; Sattler, W; Malle, E.
Selective cholesteryl ester uptake from high density lipoprotein by human first trimester and term villous trophoblast cells.
Placenta. 2003; 24(2-3):131-143
Doi: 10.1053/plac.2002.0912
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- Führende Autor*innen der Med Uni Graz
- Malle Ernst
- Wadsack Christian
- Co-Autor*innen der Med Uni Graz
- Desoye Gernot
- Frank Sasa
- Hammer Astrid
- Hirschmugl Birgit
- Levak Sanja
- Sattler Wolfgang
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- Abstract:
- As villous trophoblast does represent the contact zone between foetal and maternal tissues, the present in vitro study was aimed at investigating cholesterol supply from human high density lipoprotein subclass 3 (HDL(3)) to trophoblast cells isolated from human first trimester and term placenta. Binding of (125)I-HDL(3) was specific and saturable with similar K(d)-values for first trimester (54 microg HDL(3)-protein/ml) and term villous trophoblast cells (29 microg HDL(3)-protein/ml). The cell-association of (125)I-HDL(3) was 3-fold higher for term trophoblast cells while the specific cell-association of [(3)H]cholesterol ester(CE)-labelled HDL(3) was higher for first trimester trophoblast preparations. As a consequence, first trimester trophoblast cells have a pronounced capacity for selective CE-uptake from HDL(3). Competition experiments with native and oxidized low-density lipoprotein as well as cAMP-mediated stimulation of cell-association of [(3)H]CE-HDL(3) in both trophoblast preparations suggested the scavenger receptor class B, type I (SR-BI) as a likely receptor mediating this pathway. SR-BI m RNA could be identified by RT-PCR and Northern blot experiments in both trophoblast preparations. Western blot analysis and immunocytochemistry revealed high expression of SR-BI in first trimester trophoblast. A polyclonal antiserum raised against murine SR-BI significantly decreased cell-association of [(3)H]CE-HDL(3) in trophoblast cells. We conclude that human first trimester and term trophoblast cells express SR-BI which could serve as an efficient route for supplying cholesterol esters from maternal lipoproteins to foetal tissues.
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- Adult -
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- Antigens, CD36 - genetics Antigens, CD36 - metabolism
- Binding, Competitive -
- Blotting, Western -
- CHO Cells -
- Cell Adhesion - drug effects
- Cholesterol Esters - metabolism
- Chorionic Villi - drug effects Chorionic Villi - metabolism
- Cricetinae -
- Cyclic AMP - pharmacology
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- Humans -
- Labor, Obstetric -
- Lipoproteins, HDL - metabolism
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- Pregnancy Trimester, First -
- RNA, Messenger - metabolism
- Receptors, Immunologic -
- Receptors, Lipoprotein -
- Receptors, Scavenger -
- Reverse Transcriptase Polymerase Chain Reaction -
- Scavenger Receptors, Class B -
- Trophoblasts - cytology Trophoblasts - drug effects Trophoblasts - metabolism