Medizinische Universität Graz - Research portal
Selected Publication:
Bauernhofer, T; Kuss, I; Henderson, B; Baum, AS; Whiteside, TL.
Preferential apoptosis of CD56dim natural killer cell subset in patients with cancer.
EUR J IMMUNOL. 2003; 33: 119-124.
Doi: 10.1002/immu.200390014
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Bauernhofer Thomas
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- Abstract:
- Natural killer (NK) cells (CD56(+)/CD3(-)) in the circulation of cancer patients were reported to have low NK activity and undergo spontaneous apoptosis. A possible relationship between apoptosis and impaired NK activity was studied by Annexin V-binding and NK-cell assays performed with peripheral blood mononuclear cells of patients with head and neck cancer (HNC), breast cancer (BC) and normal controls (NC). Cells stained with Annexin V (Anx) and antibodies to CD56, CD3, CD95, CD25, CD122 or CD132 were examined by flow cytometry. NK activity was tested against K562 targets in 4-h (51)Cr-release assays. The ratio of CD56(dim)/CD56(bright) NK cells was significantly different in patients vs. controls (10 vs. 16; p<0.01). A significantly greater percentage of CD56(dim) NK cells bound Anx in HNC patients (27+/-17%, median +/- SD) or BC (46+/-18%) than in NC (15+/-18%, p<0.04 and p<0.0002, respectively). CD56(dim) NK cells were preferentially targeted for apoptosis. NK activity was significantly lower in patients with HNC and BC than in NC (p<0.009). An inverse correlation between NK activity and the percent of Anx(+)CD56(dim) NK cells was observed in cancer patients (p =0.002) but not in NC. In patients, circulating CD56(dim) NK cells were targeted for apoptosis, leading to low levels of NK activity.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Annexin A5 - metabolism
- Antigens, CD56 - metabolism
- Apoptosis - metabolism
- Female - metabolism
- Humans - metabolism
- Killer Cells, Natural - metabolism
- Lymphocyte Subsets - metabolism
- Male - metabolism
- Middle Aged - metabolism
- Neoplasms - immunology
- Protein Binding - immunology
- Receptors, Interleukin-2 - metabolism
- Research Support, Non-U.S. Gov't - metabolism
- Research Support, U.S. Gov't, P.H.S. - metabolism
- Tumor Cells, Cultured - metabolism
- Find related publications in this database (Keywords)
- Annexin V
- apoptosis
- NK cell
- cytotoxicity
- IL-2 receptor