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Selected Publication:
Bräsen, JH; Häkkinen, T; Malle, E; Beisiegel, U; Ylä-Herttuala, S.
Patterns of oxidized epitopes, but not NF-kappa B expression, change during atherogenesis in WHHL rabbits.
Atherosclerosis. 2003; 166(1):13-21
Doi: 10.1016%2FS0021-9150%2802%2900130-2
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- Co-authors Med Uni Graz
- Malle Ernst
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- Abstract:
- Oxidative modification of lipoproteins plays an important role in atherogenesis. We investigated a variety of different oxidatively modified epitopes (malondialdehyde (MDA)-2, hydroxynonenal (HNE)-7, peroxynitrite, hypochlorite, EO-6) in parallel and compared normal vessel wall, early and advanced atherosclerotic lesions in WHHL rabbits. Early atherosclerotic lesions showed abundant intracellular staining in macrophages for all ox-epitopes, apo B and apo E; advanced lesions showed a more prominent peri- and extracellular staining for ox-epitopes, which tended to colocalize more with apo B than apo E. Hypochlorite-modified epitopes showed intense staining in all types of lesions, followed by MDA-2. Early and advanced atherosclerotic lesions differed significantly in that early stages revealed abundant cellular positivity for EO-6 and weak staining for HNE-7 modified proteins whereas the opposite was observed in advanced lesions. Nuclear factor-kappa B (NF-kappa B) was nearly exclusively detected in macrophages with no difference between early and advanced lesions. We conclude that hypochlorite-modified epitopes are abundantly present at all stages of atherogenesis. EO-6 might be a marker for early, HNE-7 a marker for advanced lesions. Colocalization of ox-epitopes with apolipoproteins further supports that oxidation of lipoproteins is one of the key mechanisms in atherogenesis. Chronic stable expression and activation of NF-kappa B could be a useful target for therapeutic interventions.
- Find related publications in this database (using NLM MeSH Indexing)
- Aldehydes - metabolism
- Animals - metabolism
- Arteriosclerosis - immunology
- Epitopes - metabolism
- Hypochlorous Acid - metabolism
- Immunohistochemistry - metabolism
- Malondialdehyde - metabolism
- Nitrates - metabolism
- Oxidation-Reduction - metabolism
- Rabbits - metabolism
- Find related publications in this database (Keywords)
- atherosclerosis
- lipoprotein oxidation
- modified lipoproteins
- WHHL rabbit
- pathology
- immunohistochemistry