Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

Lang, R; Song, PI; Legat, FJ; Lavker, RM; Harten, B; Kalden, H; Grady, EF; Bunnett, NW; Armstrong, CA; Ansel, JC.
Human corneal epithelial cells express functional PAR-1 and PAR-2.
Invest Ophthalmol Vis Sci. 2003; 44(1):99-105 Doi: 10.1167/iovs.02-0357 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

Co-authors Med Uni Graz: Legat Franz
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: The objective of this study was to examine whether HCECs express functional proteinase-activated receptor (PAR)-1 and -2 and evaluate the effects of receptor activation on corneal epithelial cell proinflammatory cytokine production. Expression of PAR-1 and -2 mRNAs was determined by RT-PCR in cultured primary human corneal epithelial cells (HCECs) and the human corneal epithelial cell line HCE-T. Localization of PAR-1 and -2 in whole normal human corneas was determined by immunofluorescence with PAR-1 and -2 antibodies. The functional competence of PAR-1 and -2 in corneal epithelial cells was assessed by measuring the rapid induction of intracellular [Ca(2+)] in response to thrombin, trypsin, and specific receptor-activating peptides derived from the tethered ligands of the PAR receptors. HCE-T expression of cytokines (IL-6, IL-8, and TNFalpha) in response to activation of PAR-1 and -2 was measured by quantitative RT-PCR and ELISA. Functional PAR-1 and -2 were expressed in both HCECs and HCE-T cells. Immunoreactivity for PAR-1 and -2 was detected in the outer epithelial layer of the cornea in whole human corneal sections. Activation of PAR-1 and -2 led to upregulation in HCE-T cells of both expression of mRNA and secretion of the proinflammatory cytokines IL-6, IL-8, and TNFalpha. The results show for the first time that functional PAR-1 and -2 are present in human cornea. Activation of these receptors results in the production of various corneal epithelial cell proinflammatory cytokines. These observations indicate that PAR-1 and -2 may play an important role in modulating corneal inflammatory and wound-healing responses. These receptors may be useful therapeutic targets in several corneal disease processes.
Find related publications in this database (using NLM MeSH Indexing): Base Sequence -; Calcium - metabolism; Cell Line -; Cornea - metabolism; Cytokines - biosynthesis; DNA Primers - chemistry; Enzyme-Linked Immunosorbent Assay -; Epithelial Cells - metabolism; Fluorescent Antibody Technique, Indirect -; Humans -; Molecular Sequence Data -; RNA, Messenger - metabolism; Receptor, PAR-1 -; Receptor, PAR-2 -; Receptors, Thrombin - genetics; Receptors, Thrombin - metabolism; Reverse Transcriptase Polymerase Chain Reaction -; Thrombin - pharmacology; Trypsin - pharmacology; Up-Regulation -