Medizinische Universität Graz - Research portal
Selected Publication:
Rinner, I; Felsner, P; Hofer, D; Globerson, A; Schauenstein, K.
Characterization of the spontaneous apoptosis of rat thymocytes in vitro.
Int Arch Allergy Immunol. 1996; 111(3):230-237
Doi: 10.1159/000237372
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- Co-authors Med Uni Graz
- Schauenstein Konrad
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- Abstract:
- Unlike splenic or blood lymphocytes, rat thymocytes spontaneously undergo continuously increasing apoptosis during culture. In this study we characterized apoptotic thymus cells of rats according to cell size, nuclear dye binding and surface marker expression. Furthermore, the effects of cell density in culture, the age of the donor animals, glucocorticoids, and inhibition of protein synthesis were studied. It was found that: (1) apoptotic rat thymocytes are recognized in flow cytometry as small, acridine orange low, CD4low, CD8high cells; (2) the rate of apoptosis is dependent on the cell density in the culture in a biphasic manner; (3) thymic apoptosis increases with age of the donor animal in fresh, as well as in 24-hour cultivated cell suspensions; (4) neither adrenalectomy nor in vivo or in vitro treatment with the glucocorticoid antagonist RU 38486 influenced spontaneous apoptosis of thymocytes, and (5) inhibition of protein synthesis, which decreases apoptosis induced by corticosterone, had no effect on spontaneous apoptosis of thymocytes.
- Find related publications in this database (using NLM MeSH Indexing)
- Acridine Orange - metabolism
- Adrenalectomy - metabolism
- Aging - physiology
- Animals - physiology
- Anti-Inflammatory Agents - pharmacology
- Apoptosis - drug effects
- CD4-Positive T-Lymphocytes - drug effects
- CD8-Positive T-Lymphocytes - drug effects
- Cell Count - drug effects
- Cell Survival - drug effects
- Cells, Cultured - drug effects
- Corticosterone - pharmacology
- Cycloheximide - pharmacology
- DNA Damage - drug effects
- Flow Cytometry - drug effects
- Fluorescent Dyes - metabolism
- Hormone Antagonists - pharmacology
- Male - pharmacology
- Mifepristone - pharmacology
- Protein Synthesis Inhibitors - pharmacology
- Rats - pharmacology
- Rats, Sprague-Dawley - pharmacology
- Thymus Gland - cytology
- Find related publications in this database (Keywords)
- Apoptosis
- Thymocytes
- Rat
- Glucocorticoid
- Aging