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Selected Publication:
Schuller-Petrovic, S; Stessel, H; Brunner, F.
Ca2+ mobilization in saphenous vein smooth muscle cells derived from patients with primary varicosity.
Eur J Clin Invest. 2002; 32(9):649-656
Doi: 10.1046%2Fj.1365-2362.2002.01023.x
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Schuller-Petrovic Sanja
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- Abstract:
- BACKGROUND: Human primary varicosity is associated with 'weakness' of the vein wall. We investigated whether the reduced responsiveness of varicose veins to physiological vasoconstrictors might result from impaired Ca2+ mobilization in venous smooth muscle. MATERIALS AND METHODS: The hypothesis was tested in cells derived from phenotypically different vein segments that were obtained from the inguinal saphenous vein (tissue with incompetent valves), the distal portion of the long saphenous vein just above the medial ankle (clinically healthy tissue), and from a tributary to the long saphenous vein just below the knee (incompetent and overtly varicose tissue). Saphenous vein from patients undergoing cardiac surgery served as control. Cytosolic free Ca2+ levels ([Ca2+]i) were determined with the fura-2 method in cultured medial smooth muscle cells of third to sixth passage (21-23 measurements per tissue derived from five controls and seven patients). RESULTS: Angiotensin II (10 nmol L-1 to 10 mumol L-1) induced a significantly (P < 0.05) smaller rise in [Ca(2+)1i response in cells derived from incompetent or varicose segments (approximatley 70 nmol L-1) than in cells derived from clinically healthy vein (approximately 130 nmol L-1) or controls (approximately 170 nmol L-1). Likewise, the effect of endothelin-1 (100 nmol L-1) on [Ca2+]i was considerably less in cells derived from segments with incompetent valves or from varicose vessel segments than in cells derived from control patients (P < 0.05). In organ baths, endothelium-denuded strips of varicose vessels contracted significantly less in response to these agonists than clinically healthy segments from the same patient. CONCLUSIONS: The reduced contractility of diseased human varicose veins in response to angiotensin II and endothelin-1 involves impaired Ca2+ mobilization.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Angiotensin II - pharmacology
- Bradykinin - pharmacology
- Calcium - analysis
- Calcium Signaling - drug effects
- Case-Control Studies - drug effects
- Cells, Cultured - drug effects
- Cytosol - chemistry
- Dose-Response Relationship, Drug - chemistry
- Endothelin-1 - pharmacology
- Female - pharmacology
- Humans - pharmacology
- Male - pharmacology
- Middle Aged - pharmacology
- Muscle Contraction - drug effects
- Muscle, Smooth, Vascular - drug effects
- Saphenous Vein - drug effects
- Thrombin - pharmacology
- Varicose Veins - metabolism
- Vasoconstrictor Agents - pharmacology
- Find related publications in this database (Keywords)
- angiotensin II
- cytosolic free Ca2+
- endothelin-1
- fura-2
- human primary varicosis
- saphenous vein contraction