Medizinische Universität Graz - Research portal
Selected Publication:
Brantner, AH; Quehenberger, F; Chakraborty, A; Polligger, J; Sosa, S; Della Loggia, R.
HET-CAM bioassay as in vitro alternative to the croton oil test for investigating steroidal and non-steroidal compounds.
ALTEX. 2002; 19(2):51-56
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Quehenberger Franz
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- Abstract:
- In this study the irritation phenomena at the chorioallantoic membrane of incubated hen's eggs as an in vitro model (HET-CAM assay) were investigated in comparison to the in vivo croton oil test by including hydrocortisone, indomethacin, phenylbutazone, acetylsalicylic acid, rutin, quercetin, apigenin, and p-coumaric acid as steroidal and non-steroidal test substances. For the first time the two methods were compared in a valid way with the perspective of a realistic reduction of animal experiments. It should be investigated whether an in vitro-in vivo correlation exists and, if there is any possibility, to replace the in vivo model by an in vitro test system. Both bioassays were able to demonstrate the anti-inflammatory potency of the constituents tested. The determination of the anti-inflammatory activity of all compounds in the two test systems showed individual trends of inhibitory effects. However, the in vitro HET-CAM test was much more sensitive in comparison to the in vivo croton oil test. The croton oil test gave dose-effect correlations in the anti-inflammatory substances investigated. The modified HET-CAM assay did not provide clear dose-effect ratios. The HET-CAM assay is an inexpensive test being easy to manage after a short practical training. Because of its sensitivity the HET-CAM assay could be considered a suitable tool for qualitative testing of the anti-inflammatory activity of substances if no appropriate dose-effect curves are required. From these results it can be concluded that the different courses of the dose-effect curves may be primarily due to different mechanisms of action.
- Find related publications in this database (using NLM MeSH Indexing)
- Allantois - drug effects
- Animal Testing Alternatives - drug effects
- Animals - drug effects
- Anti-Inflammatory Agents, Non-Steroidal - pharmacology
- Biological Assay - methods
- Chick Embryo - methods
- Chorion - drug effects
- Croton Oil - drug effects
- Inflammation - drug effects
- Reproducibility of Results - drug effects
- Sensitivity and Specificity - drug effects
- Steroids - analysis
- Find related publications in this database (Keywords)
- anti-inflammatory test systems
- HET-CAM assay
- croton oil test