Medizinische Universität Graz - Research portal
Selected Publication:
Lamprecht, G; Gruber, L; Stoschitzky, K; Lindner, W.
Enantioselective analysis of (R)- and (S)-carvedilol in human plasma by high-performance liquid chromatography
CHROMATOGRAPHIA 2002 56: S35-S29.
Doi: 10.1007/BF02494109
Web of Science
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Stoschitzky Kurt
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- An HPLC column-switching method has been developed and validated for the enantioselective determination of (R)- and (S)-carvedilol in human plasma. Sample preparation was performed either off-line, by extraction with trichloromethane and back-extraction into 0.01 M aqueous citric acid which was injected on to a LiChrosorb RP 8 column, or on-line, by injecting diluted (0.1 M formic acid) plasma on to a LiChrosorb ADS column. In both instances separation was performed by gradient elution and on-line transfer of the fraction containing the carvedilol on to an enantioselective Teicoplanin column. The enantiomers of carvedilol were separated isocratically by use of methanol-acetonitrile-triethylammonium acetate, 70:30:0.05 (v/v/w), as mobile phase. With fluorescence detection the limits of quantitation were 0.30 ng mL(-1) for (R)-carvedilol and 0.26 ng mL(-1) for (S)-carvedilol; these were sufficient to enable investigation of the effect of exercise on plasma concentrations of (R)- and (S)-carvedilol after oral administration of either the racemate or the pure enantiomers.Although the operating conditions were optimized for sample preparation by on-line deproteination on a LiChrospher RP 18 ADS column, the complete method was insufficiently rugged for analysis of large numbers of plasma samples - the enantioselectivity of the Teicoplanin column deteriorated too rapidly because of the transfer of enantioselectivity-poisoning interferences which could not be suppressed sufficiently In contrast the liquid-liquid sample-extraction procedure combined with column switching resulted in a analytical method with long-term stability.
- Find related publications in this database (Keywords)
- column liquid chromatography
- enantiomer separation
- column switching
- teicoplanin column
- carvedilol