Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Kollroser, M; Schober, C.
Simultaneous determination of seven tricyclic antidepressant drugs in human plasma by direct-injection HPLC-APCI-MS-MS with an ion trap detector.
Ther Drug Monit. 2002; 24(4):537-544
Doi: 10.1097%2F00007691-200208000-00013
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- Führende Autor*innen der Med Uni Graz
- Kollroser Manfred
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- Abstract:
- A sensitive and specific direct-injection high-performance liquid chromatography-atmospheric pressure chemical ionization tandem mass-spectrometry (HPLC-APCI-MS-MS) method has been developed for the rapid identification and quantitation of seven tricyclic antidepressants-amitriptyline, nortriptyline, doxepin, dosulepin, dibenzepin, opipramol, and melitracen-in human plasma. After the addition of the internal standard lofepramine and dilution with 0.1% formic acid, plasma samples were injected into the LC-MS-MS system. Proteins and other large biomolecules were removed during an on-line sample cleanup using an Oasis extraction column (1 x 50 mm, ID, 30 microm) with a 100% aqueous mobile phase at a flow rate of 4 mL/min. The extraction column was subsequently brought in-line with the analytical column by automatic valve switching. Analytes were separated on a 5-microm Symmetry C18 (Waters) analytical column (3.0 x 150 mm, ID) using a step gradient of acetonitrile-0.1% formic acid at a flow rate of 0.6 mL/min. The total analysis time was only 12 minutes per sample. The interday and intraday coefficients of variation for all compounds were
- Find related publications in this database (using NLM MeSH Indexing)
- Antidepressive Agents, Tricyclic - blood
- Atmospheric Pressure - blood
- Chromatography, High Pressure Liquid - methods
- Humans - methods
- Mass Spectrometry - methods
- Reproducibility of Results - methods
- Sensitivity and Specificity - methods
- Find related publications in this database (Keywords)
- HPLC-APCI-MS-MS
- tricyclic antidepressants
- on-line sample cleanup