Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Kollroser, M; Schober, C.
Direct-injection high performance liquid chromatography ion trap mass spectrometry for the quantitative determination of olanzapine, clozapine and N-desmethylclozapine in human plasma.
Rapid Commun Mass Spectrom. 2002; 16(13):1266-1272
Doi: 10.1002/rcm.718
Web of Science
PubMed
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- Kollroser Manfred
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- Abstract:
- A specific and sensitive direct-injection high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) method has been developed for the rapid identification and quantitative determination of olanzapine, clozapine, and N-desmethylclozapine in human plasma. After the addition of the internal standard dibenzepin and dilution with 0.1% formic acid, plasma samples were injected into the LC/MS/MS system. Proteins and other large biomolecules were removed during an online sample cleanup using an extraction column (1 x 50 mm i.d., 30 microm) with a 100% aqueous mobile phase at a flow rate of 4 mL/min. The extraction column was subsequently brought inline with the analytical column by automatic valve switching. Analytes were separated on a 5 microm Symmetry C18 (Waters) analytical column (3.0 x 150 mm) with a mobile phase of acetonitrile/0.1% formic acid (20:80, v/v) at a flow rate of 0.5 mL/min. The total analysis time was 6 min per sample. The inter- and intra-assay coefficients of variation for all compounds were <11%. By eliminating the need for extensive sample preparation, the proposed method offers very large savings in total analysis time.
- Find related publications in this database (using NLM MeSH Indexing)
- Antipsychotic Agents - blood
- Benzodiazepines - blood
- Chromatography, High Pressure Liquid - methods
- Clozapine - analogs and derivatives
- Humans - analogs and derivatives
- Pirenzepine - analogs and derivatives
- Reproducibility of Results - analogs and derivatives
- Sensitivity and Specificity - analogs and derivatives
- Spectrometry, Mass, Electrospray Ionization - methods