Medizinische Universität Graz - Research portal
Selected Publication:
Stelzl, E; Kormann-Klement, A; Haas, J; Daghofer, E; Santner, BI; Marth, E; Kessler, HH.
Evaluation of an automated sample preparation protocol for quantitative detection of hepatitis C virus RNA.
J Clin Microbiol. 2002; 40(4):1447-1450
Doi: 10.1128/JCM.40.4.1447-1450.2002
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- Leading authors Med Uni Graz
- Kessler Harald
- Stelzl Evelyn
- Co-authors Med Uni Graz
- Daghofer Elisabeth
- Haas Josef
- Marth Egon
- Santner Brigitte
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- Abstract:
- The COBAS AMPLIPREP instrument for automated sample preparation has recently been introduced. In this study, the COBAS AMPLIPREP/COBAS AMPLICOR HCV MONITOR test, which includes this new molecular device, was evaluated and compared to the COBAS AMPLICOR HCV MONITOR test, which includes a manual extraction protocol. Interassay and intra-assay variation, precision, and linearity were determined, and a total of 130 clinical specimens were investigated. For determination of interassay variation, coefficients of variation were found to be between 9 and 59% for the COBAS AMPLIPREP/COBAS AMPLICOR HCV MONITOR test and between 13 and 69% for the COBAS AMPLICOR HCV MONITOR test. For determination of intra-assay variation, coefficients of variation were found to be between 7 and 13% for the COBAS AMPLIPREP/COBAS AMPLICOR HCV MONITOR test and between 8 and 16% for the COBAS AMPLICOR HCV MONITOR test. When precision of the COBAS AMPLIPREP/COBAS AMPLICOR HCV MONITOR test was tested, all results were found to be within +/-0.5 log of the expected results. Determination of linearity resulted in a quasilinear curve over 3 logs. When clinical samples were tested with the COBAS AMPLIPREP/COBAS AMPLICOR HCV MONITOR test and compared with the COBAS AMPLICOR HCV MONITOR test, all results were found within +/-0.5 log. In conclusion, the assay, which included the new molecular device, proved to be suitable for the routine molecular laboratory. It was found to be laborsaving and easy to handle.
- Find related publications in this database (using NLM MeSH Indexing)
- Hepacivirus - isolation and purification
- Hepatitis C - diagnosis Hepatitis C - virology
- Humans -
- Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods
- RNA, Viral - blood
- Reagent Kits, Diagnostic -
- Reverse Transcriptase Polymerase Chain Reaction -
- Robotics - instrumentation Robotics - methods