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Bachleitner-Hofmann, T; Stift, A; Friedl, J; Pfragner, R; Radelbauer, K; Dubsky, P; Schüller, G; Benkö, T; Niederle, B; Brostjan, C; Jakesz, R; Gnant, M.
Stimulation of autologous antitumor T-cell responses against medullary thyroid carcinoma using tumor lysate-pulsed dendritic cells.
J Clin Endocrinol Metab. 2002; 87(3):1098-1104 Doi: 10.1210%2Fjc.87.3.1098 [OPEN ACCESS]
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Co-authors Med Uni Graz: Pfragner Roswitha
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Abstract:: Dendritic cells (DCs) have attracted wide interest because of their unique capacity to elicit primary and secondary antitumor responses. We have generated autologous tumor lysate-pulsed DCs from three patients with medullary thyroid carcinoma (MTC) and tested them for their ability to stimulate cytotoxic T-cell responses against autologous MTC tumor cells in vitro. The aim of our investigations was to evaluate the potential efficacy of DC-based immunotherapy in patients with MTC. DCs were generated from peripheral blood monocytes using GM-CSF and IL-4 (immature DCs) or GM-CSF, IL-4, and TNFalpha (mature DCs). Our results indicate that mature tumor lysate-pulsed DCs are able to elicit a human leukocyte antigen class I-restricted cytotoxic T-cell response against autologous MTC tumor cells, whereas immature tumor lysate-pulsed DCs do not stimulate significant antitumor activity. We feel that our data may be relevant for future clinical trials of active immunotherapy using tumor lysate-pulsed DCs in patients with MTC who have residual or distant disease after surgical treatment. The fact that mature DCs displayed a substantially higher capacity to stimulate autologous antitumor T-cell responses than immature DCs underlines the importance of a maturation step in immunotherapy protocols based on DCs.
Find related publications in this database (using NLM MeSH Indexing): Adult -; Aged -; Carcinoma - immunology; Cell Aging - physiology; Cell Division - physiology; Dendritic Cells - physiology; Female - physiology; Histocompatibility Antigens Class I - analysis; Humans - analysis; Male - analysis; Middle Aged - analysis; Phenotype - analysis; T-Lymphocytes, Cytotoxic - immunology; Thyroid Neoplasms - immunology; Tumor Cells, Cultured - immunology