Medizinische Universität Graz - Research portal
Selected Publication:
Artl, A; Marsche, G; Pussinen, P; Knipping, G; Sattler, W; Malle, E.
Impaired capacity of acute-phase high density lipoprotein particles to deliver cholesteryl ester to the human HUH-7 hepatoma cell line.
Int J Biochem Cell Biol. 2002; 34(4):370-381
Doi: 10.1016/S1357-2725(01)00132-7
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Leading authors Med Uni Graz
- Malle Ernst
- Co-authors Med Uni Graz
- Marsche Gunther
- Sattler Wolfgang
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The major role of native high density lipoprotein (HDL) is to carry cholesterol from peripheral tissues to the liver for bile excretion. As acute-phase (AP)-HDL has a decreased ability for cellular cholesterol efflux but an increased capacity for cholesteryl ester (CE) delivery to peripheral tissues, the interaction of AP-HDL with human hepatoma cells was studied. Binding studies to HUH-7 cells revealed saturable binding properties for HDL and AP-HDL at 4 degrees C. At 37 degrees C, specific cell-association of (125)I- and [1,2,6,7-(3)H]-cholesteryl palmitate ([(3)H]CE)-labeled lipoprotein particles was 2.2- and 1.6-fold higher for HDL indicating that total CE delivery was significantly (P<0.05) higher for HDL in comparison to AP-HDL. In parallel, selective CE uptake (the difference between total lipid uptake and holoparticle uptake) from AP-HDL was decreased compared with HDL. The fact that the capacity for cellular cholesterol efflux from HUH-7 cells is slightly impaired by AP-HDL (compared with HDL) is of support that scavenger receptor class B, type I (SR-BI), the only receptor so far known to mediate bi-directional lipid flux, might be involved in altered HUH-7 cholesterol hemostasis by AP-HDL. Our in vitro findings suggest that HDL and AP-HDL interact differently with cells of hepatic origin resulting in decreased hepatic cholesterol removal from the circulation during the AP reaction.
- Find related publications in this database (using NLM MeSH Indexing)
- Acute-Phase Proteins - metabolism
- Animals - metabolism
- Binding, Competitive - metabolism
- Biological Transport, Active - metabolism
- Carcinoma, Hepatocellular - metabolism
- Cell Membrane - metabolism
- Cholesterol Esters - metabolism
- Humans - metabolism
- Lipoproteins, HDL - metabolism
- Liver - metabolism
- Liver Neoplasms - metabolism
- Particle Size - metabolism
- Protein Binding - metabolism
- Rabbits - metabolism
- Tumor Cells, Cultured - metabolism
- Find related publications in this database (Keywords)
- acute-phase serum amyloid A
- SAA
- inflammation and cholesterol metabolism