Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Regauer, S; Reich, O; Beham-Schmid, C.
Monoclonal gamma-T-cell receptor rearrangement in vulvar lichen sclerosus and squamous cell carcinomas.
Am J Pathol. 2002; 160(3):1035-1045
Doi: 10.1016/S0002-9440(10)64924-3
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- Führende Autor*innen der Med Uni Graz
- Regauer Sigrid
- Co-Autor*innen der Med Uni Graz
- Beham-Schmid Christine
- Reich Olaf
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- Abstract:
- Risk factors for vulvar squamous cell carcinoma (SCC) are human papilloma virus (HPV) infections and lichen sclerosus (LS). The significance of monoclonal gamma-T-cell receptor (gamma-TCR) rearrangement in the lymphoid infiltrate of LS and the consequence for vulvar carcinogenesis is unknown. One hundred sixty-one biopsies of vulvar LS and SCC, with and without LS, were examined for monoclonal gamma-TCR rearrangement and HPV16 expression, and for the expression of B- and T-cell markers and fascin. Monoclonal gamma-TCR rearrangement was identified in 8 of 17 patients with LS and 11 of 21 patients with SCC arising in LS with only occasional HPV16 DNA detection. None of the 19 SCC without LS showed monoclonal gamma-TCR rearrangement, but 14 of 19 patients had strong HPV16 detection. The lichenoid infiltrate of LS with germline configuration consisted predominantly of T cells (CD8 > CD4), along with numerous B cells. However, in biopsies with monoclonally rearranged gamma-TCR, CD4-positive T cells dominated along with B cells and fascin-positive cells in the lichenoid infiltrate and in deeply located lymphocyte aggregates (LAs). These LAs additionally contained fascin-positive dendritic cells with only individual CD8, CD57, and granzyme-positive cells. LAs in biopsies with germline configuration demonstrated numerous T cells (CD8 >CD4), but only single peripheral B cells, CD57, and fascin-positive lymphocytes. Our data suggest that monoclonal gamma-TCR rearrangement is characteristic for and limited to LS and SCC arising in LS, raising the question for a LS-associated antigen. We interpret B cells, CD4-positive T cells, and fascin-expressing dendritic cells within LS as a cellular immune response to antigen or proliferating T-cell clones. The resulting local immune dysregulation in LS may provide a permissive environment for the development of a SCC.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Carcinoma, Squamous Cell - genetics
- Female - genetics
- Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor - immunology
- Humans - immunology
- Immunohistochemistry - immunology
- Lichen Sclerosus et Atrophicus - genetics
- Lymphocytes, Tumor-Infiltrating - immunology
- Middle Aged - immunology
- Polymerase Chain Reaction - immunology
- Receptors, Antigen, T-Cell, gamma-delta - genetics
- T-Lymphocyte Subsets - immunology
- Vulvar Neoplasms - genetics