Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Morrissey, SP; Deichmann, R; Syha, J; Simonis, C; Zettl, U; Archelos, JJ; Jung, S; Stodal, H; Lassmann, H; Toyka, KV; Haase, A; Hartung, HP.
Partial inhibition of AT-EAE by an antibody to ICAM-1: clinico-histological and MRI studies.
J Neuroimmunol. 1996; 69(1-2):85-93
Doi: 10.1016/0165-5728(96)00064-1
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-Autor*innen der Med Uni Graz
- Archelos-Garcia Juan-Jose
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The role of quantitative proton magnetic resonance imaging (MRI) for the evaluation of immunopathological lesions in the CNS was studied in adoptively transferred experimental allergic encephalomyelitis (AT-EAE). We utilized a recently established treatment model, inhibition of the cell adhesion molecule ICAM-1 by the monoclonal antibody 1A-29. The animals were scanned on days 3, 5 and 7 after injection of encephalitogenic T-cells, before and after bolus injection of Gd-DTPA by performing T1-measurements to assess the integrity of the blood-brain barrier (BBB). On day 7, immunohistochemistry was performed looking for T-cells, activated macrophages, and albumin staining. There was clinical evidence of partial inhibition of AT-EAE in rats treated with antibodies against ICAM-1. This finding was in line with a significantly reduced number of T-cells in the medulla. However, the number of activated macrophages and the distribution of albumin did not differ from untreated AT-EAE animals. The histological findings are in agreement with the MRI data before and after Gd-DTPA injection which were similar in treated and untreated AT-EAE rats on day 3 and 5. On day 7 after Gd-DTPA injection there was evidence of a delayed breakdown of the BBB in the treated rats. The observation of a dissociation of clinical and MRI findings, especially evidence of Gd-enhancement despite clinical improvement, may be important in the context of interpreting MRI studies in MS patients in treatment trials.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antibodies, Monoclonal -
- Blood-Brain Barrier -
- Contrast Media -
- Encephalomyelitis, Autoimmune, Experimental - prevention and control
- Female - prevention and control
- Gadolinium DTPA - prevention and control
- Immunization, Passive - prevention and control
- Intercellular Adhesion Molecule-1 - immunology
- Magnetic Resonance Imaging - immunology
- Organometallic Compounds - diagnostic use
- Pentetic Acid - analogs and derivatives
- Rats - analogs and derivatives
- Rats, Inbred Lew - analogs and derivatives
- Time Factors - analogs and derivatives
- Find related publications in this database (Keywords)
- multiple sclerosis
- experimental allergic encephalomyelitis
- magnetic resonance imaging
- blood-brain barrier
- adhesion molecules